Full-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopy

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dc.contributor.authorTurčanová, Michaelacs
dc.contributor.authorHrtoň, Martincs
dc.contributor.authorDvořák, Petrcs
dc.contributor.authorNovák, Kamilcs
dc.contributor.authorHermanová, Markétacs
dc.contributor.authorBednařík, Zdeněkcs
dc.contributor.authorPolzer, Stanislavcs
dc.contributor.authorBurša, Jiřícs
dc.coverage.issue1cs
dc.coverage.volume2021cs
dc.date.accessioned2021-12-07T15:54:08Z
dc.date.available2021-12-07T15:54:08Z
dc.date.issued2021-11-28cs
dc.description.abstractA novel method for semiautomated assessment of directions of collagen fibers in soft tissues using histological image analysis is presented. It is based on multiple rotated images obtained via polarized light microscopy without any additional components, i.e., with just two polarizers being either perpendicular or nonperpendicular (rotated). This arrangement breaks the limitation of 90° periodicity of polarized light intensity and evaluates the in-plane fiber orientation over the whole 180° range accurately and quickly. After having verified the method, we used histological specimens of porcine Achilles tendon and aorta to validate the proposed algorithm and to lower the number of rotated images needed for evaluation. Our algorithm is capable to analyze 5·105 pixels in one micrograph in a few seconds and is thus a powerful and cheap tool promising a broad application in detection of collagen fiber distribution in soft tissues.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBioMed Research International. 2021, vol. 2021, issue 1, p. 1-13.en
dc.identifier.doi10.1155/2021/6879765cs
dc.identifier.issn2314-6141cs
dc.identifier.other175037cs
dc.identifier.urihttp://hdl.handle.net/11012/203098
dc.language.isoencs
dc.publisherHindawics
dc.relation.ispartofBioMed Research Internationalcs
dc.relation.urihttps://www.hindawi.com/journals/bmri/2021/6879765/cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2314-6141/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectsoft tissueen
dc.subjecthistological sliceen
dc.subjectcollagenen
dc.subjectfiber directionen
dc.subjectpolarized light microscopyen
dc.subjecthistogramen
dc.titleFull-Range Optical Imaging of Planar Collagen Fiber Orientation Using Polarized Light Microscopyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-175037en
sync.item.dbtypeVAVen
sync.item.insts2022.04.04 16:59:37en
sync.item.modts2022.04.04 16:15:04en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav mechaniky těles, mechatroniky a biomechanikycs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Ústav fyzikálního inženýrstvícs
thesis.grantorVysoké učení technické v Brně. . Fakultní nemocnice u sv. Anny v Brněcs
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Ústav mechaniky těles, mechatroniky a biomechaniky