Combination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophiles

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dc.contributor.authorNováčková, Ivanacs
dc.contributor.authorKouřilová, Xeniecs
dc.contributor.authorMrázová, Kateřinacs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorKalina, Michalcs
dc.contributor.authorKrzyzanek, Vladislavcs
dc.contributor.authorKoller, Martincs
dc.contributor.authorObruča, Stanislavcs
dc.coverage.issue9cs
dc.coverage.volume14cs
dc.date.accessioned2022-07-22T14:52:17Z
dc.date.available2022-07-22T14:52:17Z
dc.date.issued2022-04-26cs
dc.description.abstractProduction of polyhydroxyalkanoates (PHA), microbial biopolyesters, employing extremophilic microorganisms is a very promising concept relying on robustness of such organisms against microbial contamination, which provides numerous economic and technological benefits. In this work, we took advantage of the natural susceptibility of halophilic and thermophilic PHA producers to hypotonic lysis and we developed a simple and robust approach enabling effective isolation of PHA materials from microbial cells. The method is based on the exposition of microbial cells to hypotonic conditions induced by the diluted solution of sodium dodecyl sulfate (SDS) at elevated temperatures. Such conditions lead to disruption of the cells and release of PHA granules. Moreover, SDS, apart from its cell-disruptive function, also solubilizes hydrophobic components, which would otherwise contaminate PHA materials. The purity of obtained materials, as well as the yields of recovery, reach high values (values of purity higher than 99 wt.%, yields close to 1). Furthermore, we also focused on the removal of SDS from wastewater. The simple, inexpensive, and safe technique is based on the precipitation of SDS in the presence of KCl. The precipitate can be simply removed by decantation or centrifugation. Moreover, there is also the possibility to regenerate the SDS, which would substantially improve the economic feasibility of the process.en
dc.formattextcs
dc.format.extent1-16cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPolymers. 2022, vol. 14, issue 9, p. 1-16.en
dc.identifier.doi10.3390/polym14091761cs
dc.identifier.issn2073-4360cs
dc.identifier.other178534cs
dc.identifier.urihttp://hdl.handle.net/11012/208193
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofPolymerscs
dc.relation.urihttps://www.mdpi.com/2073-4360/14/9/1761cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4360/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyhydroxyalkanoate (PHA)en
dc.subjectsodium dodecyl sulfate (SDS)en
dc.subjectPHA isolationen
dc.subjectextremophilesen
dc.subjecthalophilesen
dc.subjectthermophilesen
dc.subjectHalomonas halophilaen
dc.subjectSchlegelella thermodepolymeransen
dc.titleCombination of Hypotonic Lysis and Application of Detergent for Isolation of Polyhydroxyalkanoates from Extremophilesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-178534en
sync.item.dbtypeVAVen
sync.item.insts2023.02.20 12:50:54en
sync.item.modts2023.02.20 12:12:36en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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