Upgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heater

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dc.contributor.authorSkopalík, Josefcs
dc.contributor.authorSekora, Jiřícs
dc.contributor.authorPešl, Martincs
dc.contributor.authorBébarová, Markétacs
dc.contributor.authorŠvecová, Olgacs
dc.contributor.authorParák, Tomášcs
dc.contributor.authorČmiel, Vratislavcs
dc.contributor.authorProvazník, Ivocs
dc.contributor.authorJeklová, Editacs
dc.contributor.authorMašek, Josefcs
dc.coverage.issue4cs
dc.coverage.volume50cs
dc.date.accessioned2021-12-14T15:55:52Z
dc.date.available2021-12-14T15:55:52Z
dc.date.issued2021-03-01cs
dc.description.abstractBiological experiments involving isolated organs and tissues demand precise temperature monitoring and regulation. An automatic temperature control system was proposed and optimised on real isolated swine hearts and the prototype is described in this work. The traditional Langendorff apparatus consists of a heart holder, a reservoir of perfusion solution flowing to aortic cannula and a heating bath allowing passive heat transfer to the reservoir of perfusion solution. The commercial infrared camera FLIR T62101 was added to this basic set-up and used for very precise monitoring of the temperature kinetic of the organ and connected with an electronic feedback loop, which allowed real-time and precise regulation of heat transfer from the heating bath to the perfusion solution and in turn indirectly to the heart tissue. This provides real time control and active regulation of the myocardial tissue temperature. The infrared camera was tested in several modes and several variants of detection were optimised for ideal measurement of the region of interest of the ex vivo organ. The kinetics of the temperature changes and temperature stability of the tissue were recorded and calibrated by external electronic thermometers (type Pt100, inserted in tissue). The time lapse from the hang-up of the hypo termed organ (30 °C) until optimal warming (37 °C) was less than eight minutes in the final instrument prototype. The final stability of the 37 °C tissue temperature was approved; the temperature fluctuation of left ventricle tissue was characterised as 36.8 ± 0.5 °C. This upgraded traditional instrument could be used in specific preclinical and clinical transplantation and analytical projects in future.en
dc.formattextcs
dc.format.extent137-141cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationLékař a technika. 2021, vol. 50, issue 4, p. 137-141.en
dc.identifier.doi10.14311/CTJ.2020.4.03cs
dc.identifier.issn0301-5491cs
dc.identifier.other173201cs
dc.identifier.urihttp://hdl.handle.net/11012/203210
dc.language.isoencs
dc.publisherCzech Society for Biomedical Engineering and Medical Informaticscs
dc.relation.ispartofLékař a technikacs
dc.relation.urihttps://ojs.cvut.cz/ojs/index.php/CTJ/article/view/7126cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0301-5491/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectisolated hearten
dc.subjectheart perfusionen
dc.subjecttissue thermostabilityen
dc.subjectthermo controlleren
dc.subjectIR thermometryen
dc.titleUpgrade of The Langendorff Apparatus Using The Infrared Thermo-Control System and An Intelligent Heateren
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-173201en
sync.item.dbtypeVAVen
sync.item.insts2021.12.14 16:55:52en
sync.item.modts2021.12.14 16:15:11en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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