A Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assays

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dc.contributor.authorFialová, Lenkacs
dc.contributor.authorLangová, Denisacs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue18cs
dc.coverage.volume25cs
dc.date.issued2020-09-25cs
dc.description.abstractFood fraud has been and still is a problem in the food industry. It is detectable by several approaches, such as high performance liquid chromatography (HPLC), chemometric assays, or DNA-based techniques, each with its own drawbacks. This work addresses one major drawback of DNA-based methods, in particular their sensitivity to inhibitors contained in particular matrices from which DNA is isolated. We tested five commercial kits and one in-house method characterized by different ways of sample homogenization and DNA capture and purification. Using these methods, DNA was isolated from 10 different fruit species commonly used in plant-based foodstuffs. The quality of the DNA was evaluated by UV-VIS spectrophotometry. Two types of qPCR assays were used for DNA quality testing: (i) Method specific for plant ITS2 region, (ii) methods specific for individual fruit species. Based mainly on the results of real-time PCR assays, we were able to find two column-based kits and one magnetic carrier-based kit, which consistently provided fruit DNA isolates of sufficient quality for PCR-based assays useful for routine analysis and identification of individual fruit species in food products.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULES. 2020, vol. 25, issue 18, p. 1-15.en
dc.identifier.doi10.3390/molecules25184317cs
dc.identifier.issn1420-3049cs
dc.identifier.orcid0000-0002-7391-305Xcs
dc.identifier.orcid0000-0002-2929-8491cs
dc.identifier.orcid0000-0002-2095-1837cs
dc.identifier.other165467cs
dc.identifier.researcheridA-6897-2016cs
dc.identifier.scopus6603465110cs
dc.identifier.urihttp://hdl.handle.net/11012/195713
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMOLECULEScs
dc.relation.urihttps://www.mdpi.com/1420-3049/25/18/4317cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1420-3049/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectDNA isolationen
dc.subjectreal-time PCRen
dc.subjectcommercial kiten
dc.subjectred fruiten
dc.subjectPrunusen
dc.subjecttropical fruiten
dc.titleA Comparative Study of Some Procedures for Isolation of Fruit DNA of Sufficient Quality for PCR-Based Assaysen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-165467en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:37en
sync.item.modts2025.01.17 16:42:01en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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