The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms

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dc.contributor.authorPorubiaková, Otíliacs
dc.contributor.authorBohálová, Natáliacs
dc.contributor.authorInga, Albertocs
dc.contributor.authorVadovičová, Natáliacs
dc.contributor.authorCoufal, Jancs
dc.contributor.authorFojta, Miroslavcs
dc.contributor.authorBrázda, Václavcs
dc.coverage.issue1cs
dc.coverage.volume21cs
dc.date.issued2020-01-14cs
dc.description.abstractp53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis and senescence. The human TP52 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure in the transactivation potential of full-lenght and N-terminal truncated p43alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53alpha isoforms.en
dc.formattextcs
dc.format.extent1-15cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2020, vol. 21, issue 1, p. 1-15.en
dc.identifier.doi10.3390/ijms21010127cs
dc.identifier.issn1422-0067cs
dc.identifier.orcid0000-0001-9940-0941cs
dc.identifier.orcid0000-0003-2837-4226cs
dc.identifier.other159576cs
dc.identifier.researcheridF-9582-2011cs
dc.identifier.urihttp://hdl.handle.net/11012/189388
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttps://www.mdpi.com/1422-0067/21/1/127cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectp53 protein protein-DNA interaction transactivatin potentailen
dc.titleThe influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoformsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-159576en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:03en
sync.item.modts2025.01.17 15:29:08en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Fakulta chemickács
thesis.grantorVysoké učení technické v Brně. . Akademie věd ČRcs
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