Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarctica

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dc.contributor.authorKrálová, Stanislavacs
dc.contributor.authorBusse, Hans-Jürgencs
dc.contributor.authorBezdíček, Matějcs
dc.contributor.authorSandoval-Powers, Megancs
dc.contributor.authorNykrýnová, Markétacs
dc.contributor.authorStaňková, Evacs
dc.contributor.authorKrsek, Danielcs
dc.contributor.authorSedláček, Ivocs
dc.coverage.issue10cs
dc.coverage.volume12cs
dc.date.accessioned2021-10-30T14:54:27Z
dc.date.available2021-10-30T14:54:27Z
dc.date.issued2021-10-22cs
dc.description.abstractDespite unfavourable Antarctic conditions, such as cold temperatures, freeze-thaw cycles, high ultraviolet radiation, dryness and lack of nutrients, microorganisms were able to adapt and surprisingly thrive in this environment. In this study, eight cold-adapted Flavobacterium strains isolated from a remote Antarctic island, James Ross Island, were studied using polyphasic taxonomic approach to determine their taxonomic position. Phylogenomic analyses based on 16S rRNA gene and 92 core genes clearly showed that these strains formed two distinct phylogenetic clusters comprising three and five strains, with average nucleotide identities significantly below 90 % in between both proposed species as well as between their closest phylogenetic relatives. Phenotyping revealed a unique pattern of biochemical and physiological characteristics enabling differentiation from the closest phylogenetically related Flavobacterium spp. Chemotaxonomic analyses showed that type strains P4023T and P7388T were characterized by the major polyamine sym-homospermidine and a quinone system containing predominantly menaquinone MK-6. In the polar lipid profile phosphatidylethanolamine, an ornithine lipid and two unidentified lipids lacking a functional group were detected as major lipids. These characteristics along with fatty acid profiles confirmed, that these species belong to the genus Flavobacterium. Thorough genomic analysis revealed presence of numerous cold-inducible or cold-adaptation associated genes, such as cold-shock proteins, proteorhodopsin, carotenoid biosynthetic genes or oxidative-stress response genes. Genomes of type strains surprisingly harboured multiple prophages, with many of them predicted to be active. Genome-mining identified biosynthetic clusters in type strain genomes with majority not matching any known biosynthetic genes which indicates further research possibilities involving these psychrotrophic bacteria. Antibiotic susceptibility testing revealed multidrug-resistant phenotype that was correlated with in silico antibiotic resistance prediction. Interestingly, while typical resistance finder tools failed to detect genes responsible for antibiotic resistance, genomic prediction confirmed multidrug-resistant profile and suggested even broader resistance than tested. Results of this study confirmed and thoroughly characterized two novel psychrotrophic Flavobacterium species, for which names Flavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov. are proposed.en
dc.formattextcs
dc.format.extent1-20cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFrontiers in Microbiology. 2021, vol. 12, issue 10, p. 1-20.en
dc.identifier.doi10.3389/fmicb.2021.729977cs
dc.identifier.issn1664-302Xcs
dc.identifier.other172701cs
dc.identifier.urihttp://hdl.handle.net/11012/201796
dc.language.isoencs
dc.publisherFrontiers Media SAcs
dc.relation.ispartofFrontiers in Microbiologycs
dc.relation.urihttps://www.frontiersin.org/articles/10.3389/fmicb.2021.729977/fullcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1664-302X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectAntarcticaen
dc.subjectpsychrophilesen
dc.subjectcold-adaptationen
dc.subjectphylogenomicsen
dc.subjectsystematicsen
dc.subjectFlavobacterium flabelliforme sp. nov.en
dc.subjectFlavobacterium geliluteum sp. nov.en
dc.titleFlavobacterium flabelliforme sp. nov. and Flavobacterium geliluteum sp. nov., two multidrug-resistant psychrotrophic species isolated from Antarcticaen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172701en
sync.item.dbtypeVAVen
sync.item.insts2021.11.25 16:56:38en
sync.item.modts2021.11.25 16:15:28en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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