Phasor analysis of NADH FLIM identifies pharmacological disruptions to mitochondrial metabolic processes in the rodent cerebral cortex
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Date
2018-03-21
Authors
Gómez, Carlos A.
Sutin, Jason
Wu, Weicheng
Fu, Buyin
Uhlířová, Hana
Devor, Anna
Boas, David A.
Sakadžic, Sava
Yaseen, Mohammad Abbas
ORCID
0000-0002-0704-3148Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
PLOS
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Abstract
Investigating cerebral metabolism in vivo at a microscopic level is essential for understanding brain function and its pathological alterations. The intricate signaling and metabolic dynamics between neurons, glia, and microvasculature requires much more detailed understanding to better comprehend the mechanisms governing brain function and its disease related changes. We recently demonstrated that pharmacologically-induced alterations to different steps of cerebral metabolism can be distinguished utilizing 2-photon fluorescence lifetime imaging of endogenous reduced nicotinamide adenine dinucleotide (NADH) fluorescence in vivo. Here, we evaluate the ability of the phasor analysis method to identify these pharmacological metabolic alterations and compare the method's performance with more conventional nonlinear curve-fitting analysis. Visualization of phasor data, both at the fundamental laser repetition frequency and its second harmonic, enables resolution of pharmacologically-induced alterations to mitochondrial metabolic processes from baseline cerebral metabolism. Compared to our previous classification models based on nonlinear curve-fitting, phasor based models required fewer parameters and yielded comparable or improved classification accuracy. Fluorescence lifetime imaging of NADH and phasor analysis shows utility for detecting metabolic alterations and will lead to a deeper understanding of cerebral energetics and its pathological changes.
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Citation
PLOS ONE. 2018, vol. 13, issue 3, p. e0194578-e0194578.
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194578
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0194578
Document type
Peer-reviewed
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Published version
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en