Plasmide DNA Isolation from Bacteria and Transfection to HEK293 Cell Line
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Karmazínová, K.
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Referee
Mark
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Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologií
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Abstract
Isolation DNA is a one of the basic methods in molecular biology. There are several methods of DNA amplification and isolation. In this paper phenol-chloroform extraction of three plasmid types is used: Channelrhodopsin-2, ASAP and KIR. Seven plasmids were isolated in total. These plasmids are then validated using gel electrophoresis. Successfully isolated plasmids are then transfected to HEK293 and taken on confocal microscope 24 hours after transfection.
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Proceedings of the 21st Conference STUDENT EEICT 2015. s. 125-127. ISBN 978-80-214-5148-3
http://www.feec.vutbr.cz/EEICT/
http://www.feec.vutbr.cz/EEICT/
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Peer-reviewed
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cs