Optimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cells

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dc.contributor.authorKrobot, Štěpáncs
dc.contributor.authorMenčík, Přemyslcs
dc.contributor.authorChaloupková, Kateřinacs
dc.contributor.authorBočkaj, Jáncs
dc.contributor.authorAgócsová, Sára Vachcs
dc.contributor.authorKlusáček Rampichová, Michalacs
dc.contributor.authorHedvičáková, Věracs
dc.contributor.authorAlexy, Pavolcs
dc.contributor.authorPřikryl, Radekcs
dc.contributor.authorMelčová, Veronikacs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.accessioned2025-06-10T10:57:30Z
dc.date.available2025-06-10T10:57:30Z
dc.date.issued2024-12-18cs
dc.description.abstractBone tissue engineering requires scaffolds with three-dimensional (3D) structures that facilitate vascularization and new tissue growth. 3D printing, especially through fused deposition modeling (FDM), has emerged as an effective method for creating complex structures with high reproducibility. Early research in this area demonstrated the potential of poly(-caprolactone) (PCL) and poly(L-lactide) (PLLA) scaffolds for bone regeneration. Recently, polylactide (PLA) and polyhydroxyalkanoates (PHAs) have garnered attention for their biocompatibility and ability to support cell proliferation. Among PHAs, poly(3-hydroxybutyrate) (P3HB) shows promise due to its intrinsic biocompatibility and resorbability, making it a candidate for FDM-based scaffold fabrication. In the presented study, we aim to develop and optimize a biocompatible P3HB-based composite material for bone tissue engineering, incorporating PLA, hydroxyapatite (HA), and the plasticizer Syncroflex3114 (SN) to enhance mechanical properties and printability. This composite was processed into filaments for 3D printing and characterized through thermal, mechanical, and biological evaluations. Using a design of experiment (DoE) approach, we investigated factors such as temperature performance, warping, degradation, and strength to determine the optimal composition for use in tissue engineering. Four optimal mixture compositions fulfilling the optimization criteria of having the most suitable properties for bone tissue engineering, namely the best printability and maximum mechanical properties, were obtained. The mixtures were optimized specifically for minimum warping coefficient (0.5); maximum flexural strength (66.9 MPa); maximum compression modulus (2.4 GPa); and maximum compression modulus (2.3 GPa) with a warping coefficient of no more than 1 at the same time. In conclusion, the study shows a new possible way to effectively develop and test 3D-printed P3HB-based scaffolds with specifically optimized material properties.en
dc.formattextcs
dc.format.extent1-18cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Bioprinting. 2024, vol. 11, issue 1, p. 1-18.en
dc.identifier.doi10.36922/ijb.5175cs
dc.identifier.issn2424-8002cs
dc.identifier.orcid0000-0002-8647-2097cs
dc.identifier.orcid0000-0002-1914-8764cs
dc.identifier.orcid0000-0002-7811-9840cs
dc.identifier.orcid0000-0002-2124-6953cs
dc.identifier.other193562cs
dc.identifier.researcheridHHN-0528-2022cs
dc.identifier.researcheridE-8210-2010cs
dc.identifier.researcheridABD-4360-2020cs
dc.identifier.scopus57984865200cs
dc.identifier.scopus57204110910cs
dc.identifier.urihttps://hdl.handle.net/11012/251590
dc.language.isoencs
dc.publisherAccScience Publishingcs
dc.relation.ispartofInternational Journal of Bioprintingcs
dc.relation.urihttps://www.accscience.com/journal/IJB/articles/online_first/4158cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2424-8002/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectPoly(3-hydroxybutyrate)en
dc.subjectPolylactideen
dc.subject3D printingen
dc.subjectFused deposition modelingen
dc.subjectDesign of Experimenten
dc.subjectScaffolden
dc.subjectPrintabilityen
dc.titleOptimizing printability and mechanical properties of poly(3-hydroxybutyrate) biocomposite blends and their biological response to Saos-2 cellsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-193562en
sync.item.dbtypeVAVen
sync.item.insts2025.06.10 12:57:30en
sync.item.modts2025.06.10 12:33:21en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
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