Preparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compounds

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dc.contributor.authorUhlířová, Renatacs
dc.contributor.authorSkoumalová, Petracs
dc.contributor.authorHoová, Juliecs
dc.contributor.authorPořízka, Jaromírcs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue1cs
dc.coverage.volume324cs
dc.date.accessioned2021-01-13T16:56:48Z
dc.date.available2021-01-13T16:56:48Z
dc.date.issued2020-10-01cs
dc.description.abstractIn this study preparation and characterization of new UV-protecting systems based on liposomes/polyhydroxybutyrate (PHB) with encapsulated coffee extracts are presented. Green and roasted coffee extracts with high phenolics content, high antioxidant activity and sun protection factor (SPF) value 40–50 were used as model organic UV filters and encapsulated into liposomes and PHB-liposomes. Particle size and colloid stability was observed by dynamic light scattering and zeta-potential. Toxicity of particles was tested by MTT and LDH assay on HaCaT cell line. All prepared samples showed moderate or high encapsulation efficiency. Addition of PHB up to 50 % of lecithin led to increased size and stability. As optimal addition of 20 % PHB into liposome particles was found leading to optimum size and processing of particles, to high UV-protective effect as well as to increased colloid stability and SPF value during long-term storage. Significant differences in cell viability were found in cytotoxicity studies after exposure of keratinocytes to liposomes with different PHB content. Newly fabricated PHB-liposome particles with coffee extract were not found as toxic for HaCaT cells and in LDH test up to 12 %. These particles can act as active carriers for organic sunscreen components in combination with UV-protective effect of PHB.en
dc.formattextcs
dc.format.extent1-10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Biotechnology. 2020, vol. 324, issue 1, p. 1-10.en
dc.identifier.doi10.1016/j.btecx.2020.100021cs
dc.identifier.issn0168-1656cs
dc.identifier.other166337cs
dc.identifier.urihttp://hdl.handle.net/11012/195851
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofJournal of Biotechnologycs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S2590155920300081cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0168-1656/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectNanoparticlesen
dc.subjectPHB-liposomesen
dc.subjectStabilityen
dc.subjectCytotoxicityen
dc.subjectUV protectionen
dc.subjectCoffea arabicaen
dc.titlePreparation and characterisation of organic UV filters based on combined PHB/liposomes with natural phenolic compoundsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-166337en
sync.item.dbtypeVAVen
sync.item.insts2023.03.13 16:52:58en
sync.item.modts2023.03.13 16:14:30en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV Laboratoř ÚCHPBTcs
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