Rapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysis

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dc.contributor.authorDufková, Kristýnacs
dc.contributor.authorBezdíček, Matějcs
dc.contributor.authorJakubíčková, Markétacs
dc.contributor.authorKocmanová, Ivacs
dc.contributor.authorKubáčková, Petracs
dc.contributor.authorHanslikova, Janacs
dc.contributor.authorFejková, Karolínacs
dc.contributor.authorMayer, Jiřícs
dc.contributor.authorLengerová, Martinacs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.issued2023-01-11cs
dc.description.abstractThe Pseudomonas aeruginosa population has a nonclonal epidemic structure. It is generally composed of a limited number of widespread clones selected from a background of many rare and unrelated genotypes recombining at high frequency. Due to the increasing prevalence of nosocomial infections caused by multidrug-resistant/extensively drug-resistant (MDR/XDR) strains, it is advisable to implement infection control measures. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) are considered the gold standard methods in bacterial typing, despite being limited by cost, staff, and instrumental demands. Here, we present a novel mini-MLST scheme for P. aeruginosa rapid genotyping based on high-resolution melting analysis. Using the proposed mini-MLST scheme, 3,955 existing sequence types (STs) were converted into 701 melting types (MelTs), resulting in a discriminatory power of D=0.993 (95% confidence interval [CI],0.992 to 0.994). Whole-genome sequencing of 18 clinical isolates was performed to support the newly designed mini-MLST scheme. The clonal analysis of STs belonging to MelTs associated with international high-risk clones (HRCs) performed by goeBURST software revealed that a high proportion of the included STs are highly related to HRCs and have also been witnessed as responsible for serious infections. Therefore, mini-MLST provides a clear warning for the potential spread of P. aeruginosa clones recognized as MDR/XDR strains with possible serious outcomes.en
dc.formattextcs
dc.format.extent1-9cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicrobiology spectrum. 2023, vol. 11, issue 1, p. 1-9.en
dc.identifier.doi10.1128/spectrum.03571-22cs
dc.identifier.issn2165-0497cs
dc.identifier.orcid0000-0002-0205-6935cs
dc.identifier.other180802cs
dc.identifier.researcheridR-3181-2018cs
dc.identifier.scopus57202469794cs
dc.identifier.urihttp://hdl.handle.net/11012/209214
dc.language.isoencs
dc.publisherAmerican Society for Microbiologycs
dc.relation.ispartofMicrobiology spectrumcs
dc.relation.urihttps://journals.asm.org/doi/10.1128/spectrum.03571-22cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2165-0497/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectPseudomonas aeruginosaen
dc.subjecthigh-resolution meltingen
dc.subjectmini-MLSTen
dc.subjectmolecular epidemiologyen
dc.subjectstrain typingen
dc.titleRapid Identification of Pseudomonas aeruginosa International High-Risk Clones Based on High-Resolution Melting Analysisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-180802en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:39:56en
sync.item.modts2025.01.17 16:51:16en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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