Computational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modes

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dc.contributor.authorTučková, Terezacs
dc.contributor.authorŠiler, Martincs
dc.contributor.authorFlaes, Dirk E. Boonzajercs
dc.contributor.authorJákl, Petrcs
dc.contributor.authorTurtaev, Sergeycs
dc.contributor.authorKrátký, Stanislavcs
dc.contributor.authorHeintzmann, Rainercs
dc.contributor.authorUhlířová, Hanacs
dc.contributor.authorČižmár, Tomášcs
dc.coverage.issue23cs
dc.coverage.volume29cs
dc.date.accessioned2021-12-07T15:54:08Z
dc.date.available2021-12-07T15:54:08Z
dc.date.issued2021-11-08cs
dc.description.abstractIn imaging geometries, which employ wavefront-shaping to control the light transport through a multi-mode optical fibre (MMF), this terminal hair-thin optical component acts as a minimally invasive objective lens, enabling high resolution laser-scanning fluorescence microscopy inside living tissues at depths hardly accessible by any other light-based technique. Even in the most advanced systems, the diffraction-limited foci scanning the object across the focal plane are contaminated by a stray optical signal carrying typically few tens of % of the total optical power. The stray illumination takes the shape of a randomised but reproducible speckle, and is unique for each position of the focus. We experimentally demonstrate that the performance of imaging a fluorescent object can be significantly improved, when resulting images are computationally post-processed, utilising records of intensities of all speckle-contaminated foci used in the imaging procedure. We present two algorithms based on a regularised iterative inversion and regularised direct pseudo-inversion respectively which lead to enhancement of the image contrast and resolution. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreementen
dc.formattextcs
dc.format.extent38206-38220cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationOPTICS EXPRESS. 2021, vol. 29, issue 23, p. 38206-38220.en
dc.identifier.doi10.1364/OE.434848cs
dc.identifier.issn1094-4087cs
dc.identifier.other174094cs
dc.identifier.urihttp://hdl.handle.net/11012/203100
dc.language.isoencs
dc.publisherOptica Publishing Groupcs
dc.relation.ispartofOPTICS EXPRESScs
dc.relation.urihttps://www.osapublishing.org/oe/fulltext.cfm?uri=oe-29-23-38206&id=464465cs
dc.rights(C) Optica Publishing Groupcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1094-4087/cs
dc.subjectLIGHTen
dc.subjectTRANSFORMATIONen
dc.subjectTRANSMISSIONen
dc.subjectRESOLUTIONen
dc.subjectPHASEen
dc.titleComputational image enhancement of multimode fibre-based holographic endo-microscopy: harnessing the muddy modesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-174094en
sync.item.dbtypeVAVen
sync.item.insts2021.12.07 16:54:08en
sync.item.modts2021.12.07 16:14:30en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
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