Quantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16

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dc.contributor.authorObruča, Stanislavcs
dc.contributor.authorSedláček, Petrcs
dc.contributor.authorBenešová, Pavlacs
dc.contributor.authorKučera, Dancs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue11cs
dc.coverage.volume16cs
dc.date.accessioned2020-08-04T10:59:24Z
dc.date.available2020-08-04T10:59:24Z
dc.date.issued2016-10-28cs
dc.description.abstractWe report herein on the application of Raman spectroscopy to the rapid quantitative analysis of polyhydroxyalkanoates (PHAs), biodegradable polyesters accumulated by various bacteria. This theme was exemplified for quantitative detection of the most common member of PHAs, poly(3-hydroxybutyrate) (PHB) in Cupriavidus necator H16. We have identified the relevant spectral region (800-1800 cm(-1)) incorporating the Raman emission lines exploited for the calibration of PHB (PHB line at 1736 cm-1) and for the selection of the two internal standards (DNA at 786 cm(-1) and Amide I at 1662 cm(-1)). In order to obtain quantitative data for calibration of intracellular content of PHB in bacterial cells reference samples containing PHB amounts determined by gas chromatography from 12% to 90% (w/w) were used. Consequently, analytical results based on this calibration can be used for fast and reliable determination of intracellular PHB content during biotechnological production of PHB since the whole procedure from bacteria sampling, centrifugation, and sample preparation to Raman analysis can take about 12 min. In contrast, gas chromatography analysis takes approximately 8 h.en
dc.formattextcs
dc.format.extent1-7cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2016, vol. 16, issue 11, p. 1-7.en
dc.identifier.doi10.3390/s16111808cs
dc.identifier.issn1424-8220cs
dc.identifier.other129622cs
dc.identifier.urihttp://hdl.handle.net/11012/63734
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttp://www.mdpi.com/1424-8220/16/11/1808cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectRaman spectroscopyen
dc.subjectCupriavidus necator H16en
dc.subjectpolyhydroxyalkanoatesen
dc.titleQuantitative Raman Spectroscopy Analysis of Polyhydroxyalkanoates Produced by Cupriavidus necator H16en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-129622en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 12:59:24en
sync.item.modts2020.08.04 12:35:09en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV - laboratoř biokoloidůcs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. CMV - laboratoř biotechnologiícs
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