Bioinformatics study of the third generation sequencing platforms applied on a thermophile

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but.event.date23.04.2024cs
but.event.titleSTUDENT EEICT 2024cs
dc.contributor.authorUmair, Mohammad
dc.contributor.authorŘeháková, Veronika
dc.contributor.authorBuchtikova, Iva
dc.contributor.authorBezdicek, Matej
dc.contributor.authorObruca, Stanislav
dc.contributor.authorSedlář, Karel
dc.date.accessioned2024-07-09T07:38:39Z
dc.date.available2024-07-09T07:38:39Z
dc.date.issued2024cs
dc.description.abstractThis study compares the efficiency of Pacific Biosciences technology (PacBio) and Oxford Nanopore Technology (ONT) in sequencing, assembling, and annotating the Aneurinibacillus sp AFn2 bacterium. We aim to evaluate the performance based on contiguity, depth, and functional annotation of the resulting genome. Using ONT we generated 152,047 long reads assembling into 2 contigs with total base count of 0.4 billion which provided us efficient assembly while PacBio produced 139,701 reads, assembling into 21 contigs with a total base count of 1.4 billion. Functional annotation revealed differences in the number of coding sequences, with PacBio detecting more comprehensive gene sets than ONT. The comparative analysis done in this research shows the strengths and limitations of both the platforms, with ONT providing higher assembly contiguity and PacBio offering greater detail in genetic content. We aim to offer insights of both the sequencing technologies, guiding researchers in selecting the appropriate technology.en
dc.formattextcs
dc.format.extent202-206cs
dc.format.mimetypeapplication/pdfen
dc.identifier.citationProceedings I of the 30st Conference STUDENT EEICT 2024: General papers. s. 202-206. ISBN 978-80-214-6231-1cs
dc.identifier.isbn978-80-214-6231-1
dc.identifier.issn2788-1334
dc.identifier.urihttps://hdl.handle.net/11012/249235
dc.language.isoencs
dc.publisherVysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.relation.ispartofProceedings I of the 30st Conference STUDENT EEICT 2024: General papersen
dc.relation.urihttps://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_1.pdfcs
dc.rights© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.rights.accessopenAccessen
dc.subjectAneurinibacillusen
dc.subjectNanoporeen
dc.subjectPacBioen
dc.subjectSequencingen
dc.subjectGenome Assemblyen
dc.titleBioinformatics study of the third generation sequencing platforms applied on a thermophileen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.affiliatedInstitution.departmentFakulta elektrotechniky a komunikačních technologiícs
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