Characterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.

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dc.contributor.authorSedláček, Ivocs
dc.contributor.authorHolochová, Pavlacs
dc.contributor.authorBusse, Hans-Jürgencs
dc.contributor.authorKoublová, Vendulacs
dc.contributor.authorKrálová, Stanislavacs
dc.contributor.authorŠvec, Pavelcs
dc.contributor.authorSobotka, Romancs
dc.contributor.authorStaňková, Evacs
dc.contributor.authorPilný, Jancs
dc.contributor.authorŠedo, Ondrejcs
dc.contributor.authorSmolíková, Janacs
dc.contributor.authorSedlář, Karelcs
dc.coverage.issue4cs
dc.coverage.volume10cs
dc.date.accessioned2022-05-27T10:53:47Z
dc.date.available2022-05-27T10:53:47Z
dc.date.issued2022-03-24cs
dc.description.abstractA group of seven bacterial strains producing blue-purple pigmented colonies on R2A agar was isolated from freshwater samples collected in a deglaciated part of James Ross Island and Eagle Island, Antarctica, from 2017-2019. The isolates were psychrophilic, oligotrophic, resistant to chloramphenicol, and exhibited strong hydrolytic activities. To clarify the taxonomic position of these isolates, a polyphasic taxonomic approach was applied based on sequencing of the 16S rRNA, gyrB and lepA genes, whole-genome sequencing, rep-PCR, MALDI-TOF MS, chemotaxonomy analyses and biotyping. Phylogenetic analysis of the 16S rRNA gene sequences revealed that the entire group are representatives of the genus Massilia. The closest relatives of the reference strain P8398(T) were Massilia atriviolacea, Massilia violaceinigra, Massilia rubra, Massilia mucilaginosa, Massilia aquatica, Massilia frigida, Massilia glaciei and Massilia eurypsychrophila with a pairwise similarity of 98.6-100% in the 16S rRNA. The subsequent gyrB and lepA sequencing results showed the novelty of the analysed group, and the average nucleotide identity and digital DNA-DNA hybridisation values clearly proved that P8398(T) represents a distinct Massilia species. After all these results, we nominate a new species with the proposed name Massilia antarctica sp. nov. The type strain is P8398(T) (= CCM 8941(T) = LMG 32108(T)).en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2022, vol. 10, issue 4, p. 1-14.en
dc.identifier.doi10.3390/microorganisms10040704cs
dc.identifier.issn2076-2607cs
dc.identifier.other177721cs
dc.identifier.urihttp://hdl.handle.net/11012/204462
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/2076-2607/10/4/704/htmcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectMassiliaen
dc.subjectviolaceinen
dc.subjectpsychrophilicen
dc.subjectdescriptionen
dc.subjectwhole-genome sequencingen
dc.subjectAntarcticaen
dc.titleCharacterisation of Waterborne Psychrophilic Massilia Isolates with Violacein Production and Description of Massilia antarctica sp. nov.en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-177721en
sync.item.dbtypeVAVen
sync.item.insts2022.11.15 12:52:25en
sync.item.modts2022.11.15 12:14:27en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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