In vitro culture of leukemic cells in collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gel

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dc.contributor.authorSvozilova, Hanacs
dc.contributor.authorVojtová, Lucycs
dc.contributor.authorMatulová, Janacs
dc.contributor.authorBruknerova, Janacs
dc.contributor.authorPoláková, Veronikacs
dc.contributor.authorRadová, Lenkacs
dc.contributor.authorDoubek, Michaelcs
dc.contributor.authorPlevová, Karlacs
dc.contributor.authorPospíšilová, Šárkacs
dc.coverage.issue12cs
dc.coverage.volume12cs
dc.date.accessioned2025-04-04T11:56:52Z
dc.date.available2025-04-04T11:56:52Z
dc.date.issued2024-12-06cs
dc.description.abstractBackground: Chronic lymphocytic leukemia (CLL) is a common adult leukemia characterized by the accumulation of neoplastic mature B cells in blood, bone marrow, lymph nodes, and spleen. The disease biology remains unresolved in many aspects, including the processes underlying the disease progression and relapses. However, studying CLL in vitro poses a considerable challenge due to its complexity and dependency on the microenvironment. Several approaches are utilized to overcome this issue, such as co-culture of CLL cells with other cell types, supplementing culture media with growth factors, or setting up a three-dimensional (3D) culture. Previous studies have shown that 3D cultures, compared to conventional ones, can lead to enhanced cell survival and altered gene expression. 3D cultures can also give valuable information while testing treatment response in vitro since they mimic the cell spatial organization more accurately than conventional culture. Methods: In our study, we investigated the behavior of CLL cells in two types of material: (i) solid porous collagen scaffolds and (ii) gel composed of carboxymethyl cellulose and polyethylene glycol (CMC-PEG). We studied CLL cells' distribution, morphology, and viability in these materials by a transmitted-light and confocal microscopy. We also measured the metabolic activity of cultured cells. Additionally, the expression levels of MYC, VCAM1, MCL1, CXCR4, and CCL4 genes in CLL cells were studied by qPCR to observe whether our novel culture approaches lead to increased adhesion, lower apoptotic rates, or activation of cell signaling in relation to the enhanced contact with co-cultured cells. Results: Both materials were biocompatible, translucent, and permeable, as assessed by metabolic assays, cell staining, and microscopy. While collagen scaffolds featured easy manipulation, washability, transferability, and biodegradability, CMC-PEG was advantageous for its easy preparation process and low variability in the number of accommodated cells. Both materials promoted cell-to-cell and cell-to-matrix interactions due to the scaffold structure and generation of cell aggregates. The metabolic activity of CLL cells cultured in CMC-PEG gel was similar to or higher than in conventional culture. Compared to the conventional culture, there was (i) a lower expression of VCAM1 in both materials, (ii) a higher expression of CCL4 in collagen scaffolds, and (iii) a lower expression of CXCR4 and MCL1 (transcript variant 2) in collagen scaffolds, while it was higher in a CMC-PEG gel. Hence, culture in the material can suppress the expression of a pro-apoptotic gene ( MCL1 in collagen scaffolds) or replicate certain gene expression patterns attributed to CLL cells in lymphoid organs (low CXCR4, high CCL4 in collagen scaffolds) or blood (high CXCR4 in CMC-PEG).en
dc.formattextcs
dc.format.extent1-30cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPeerJ. 2024, vol. 12, issue 12, p. 1-30.en
dc.identifier.doi10.7717/peerj.18637cs
dc.identifier.issn2167-8359cs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.orcid0000-0002-4072-8173cs
dc.identifier.other196614cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.scopus12039667200cs
dc.identifier.urihttps://hdl.handle.net/11012/250796
dc.language.isoencs
dc.publisherPeerJcs
dc.relation.ispartofPeerJcs
dc.relation.urihttps://peerj.com/articles/18637/cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2167-8359/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectChronic lymphocytic leukemiaen
dc.subjectCLLen
dc.subject3D cultureen
dc.subjectCarboxymethyl celluloseen
dc.subjectCMCen
dc.subjectPolyethylene glycolen
dc.subjectPEGen
dc.subjectCollagenen
dc.subjectScaffoldsen
dc.subjectGelen
dc.titleIn vitro culture of leukemic cells in collagen scaffolds and carboxymethyl cellulose-polyethylene glycol gelen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-196614en
sync.item.dbtypeVAVen
sync.item.insts2025.04.04 13:56:52en
sync.item.modts2025.04.03 13:32:08en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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