Quantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Light

Simple item page
dc.contributor.authorFeith, Marekcs
dc.contributor.authorVičar, Tomášcs
dc.contributor.authorGumulec, Jaromírcs
dc.contributor.authorRaudenská, Martinacs
dc.contributor.authorGjörloff Wingren, Anettecs
dc.contributor.authorMasařík, Michalcs
dc.contributor.authorBalvan, Jancs
dc.coverage.issue7cs
dc.coverage.volume10cs
dc.date.accessioned2020-08-04T11:00:12Z
dc.date.available2020-08-04T11:00:12Z
dc.date.issued2020-04-09cs
dc.description.abstractIncreased exposition to blue light may induce many changes in cell behavior and significantly affect the critical characteristics of cells. Here we show that multimodal holographic microscopy (MHM) within advanced image analysis is capable of correctly distinguishing between changes in cell motility, cell dry mass, cell density, and cell death induced by blue light. We focused on the effect of blue light with a wavelength of 485 nm on morphological and dynamical parameters of four cell lines, malignant PC-3, A2780, G361 cell lines, and the benign PNT1A cell line. We used MHM with blue light doses 24 mJ/cm2, 208 mJ/cm2 and two kinds of expositions (500 and 1000 ms) to acquire real-time quantitative phase information about cellular parameters. It has been shown that specific doses of the blue light significantly influence cell motility, cell dry mass and cell density. These changes were often specific for the malignant status of tested cells. Blue light dose 208 mJ/cm2 × 1000 ms affected malignant cell motility but did not change the motility of benign cell line PNT1A. This light dose also significantly decreased proliferation activity in all tested cell lines but was not so deleterious for benign cell line PNT1A as for malignant cells. Light dose 208 mJ/cm2 × 1000 ms oppositely affected cell mass in A2780 and PC-3 cells and induced different types of cell death in A2780 and G361 cell lines. Cells obtained the least damage on lower doses of light with shorter time of exposition.en
dc.formattextcs
dc.format.extent1-13cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationApplied Sciences - Basel. 2020, vol. 10, issue 7, p. 1-13.en
dc.identifier.doi10.3390/app10072597cs
dc.identifier.issn2076-3417cs
dc.identifier.other163786cs
dc.identifier.urihttp://hdl.handle.net/11012/189003
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofApplied Sciences - Baselcs
dc.relation.urihttps://www.mdpi.com/2076-3417/10/7/2597cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-3417/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectholographic microscopyen
dc.subjectquantitative phase imagingen
dc.subjectblue lighten
dc.subjectcell massen
dc.subjectcell motilityen
dc.subjectcell deathen
dc.titleQuantitative Phase Dynamics of Cancer Cell Populations Affected by Blue Lighten
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-163786en
sync.item.dbtypeVAVen
sync.item.insts2020.08.13 16:58:30en
sync.item.modts2020.08.13 16:15:29en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
applsci1002597.pdf
Size:
1.93 MB
Format:
Adobe Portable Document Format
Description:
applsci1002597.pdf
Download
Collections
Ústav biomedicínského inženýrství