In-depth Bioiformatic Analyses of Human SARS-CoV-2, SARS-CoV, MERS-CoV, and Other Nidovirales Suggest Important Roles of Noncanonical Nucleic Acid Structures in Their Lifecycles

Simple item page
dc.contributor.authorBartas, Martincs
dc.contributor.authorBrázda, Václavcs
dc.contributor.authorBohálová, Natáliacs
dc.contributor.authorCantara, Alessiocs
dc.contributor.authorVolná, Adrianacs
dc.contributor.authorStachurová, Terezacs
dc.contributor.authorMalachová, Katerinacs
dc.contributor.authorBrázdová Jagelská, Evacs
dc.contributor.authorPorubiaková, Otíliacs
dc.contributor.authorCerven, Jirícs
dc.contributor.authorPečinka, Petrcs
dc.coverage.issue1cs
dc.coverage.volume11cs
dc.date.issued2020-04-03cs
dc.description.abstractNoncanonical nucleic acid structures play important roles in the regulation of molecular processess. Considering the importance of the ongoing coronavirus crisis, we decided to evaluate genomes of all coronaviruses sequences to date (stated more broadly, the order Nidovirales) to determine if they contain noncanonical nucleic acid structures. We discovered much evidence of the putative G-quadruplex sites and even much more of inverted repeats (IRs) loci, which in fact are ubiquitous along the whole genomic sequence and indicate a possible mechanism for genomic RNA packaging. The most notable enrichment if IRs was found inside 5UTR for IRs of size 12+ nucleotides, and the most notable enrichment of putative quadruplex sites (PQSs) was located befor 3UTR, inside 5UTR, and before mRNA. This indicates crucial regulatory roles for both IRs and PQSs. Moreover, we found multiple G-quadruplex binding motifs in human proteins having potential for binding of SARS-CoV-2 RNA. Noncanonical nucleic acids structures in Nidoviralesand in novel SARS-CoV-2 are therefore promising druggable structures that can be targeted and utilized in the future.en
dc.formattextcs
dc.format.extent1-29cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationFrontiers in Microbiology. 2020, vol. 11, issue 1, p. 1-29.en
dc.identifier.doi10.3389/fmicb.2020.01583cs
dc.identifier.issn1664-302Xcs
dc.identifier.orcid0000-0003-2837-4226cs
dc.identifier.orcid0000-0001-9940-0941cs
dc.identifier.other163434cs
dc.identifier.researcheridF-9582-2011cs
dc.identifier.urihttp://hdl.handle.net/11012/194859
dc.language.isoencs
dc.publisherFrontierscs
dc.relation.ispartofFrontiers in Microbiologycs
dc.relation.urihttp://www.frontiersin.org/articles/10.3389/fmicb.2020.01583/fullcs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1664-302X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcoronavirusen
dc.subjectgenomeen
dc.subjectRNAen
dc.subjectG-quadruplexen
dc.subjectinverted repeatsen
dc.titleIn-depth Bioiformatic Analyses of Human SARS-CoV-2, SARS-CoV, MERS-CoV, and Other Nidovirales Suggest Important Roles of Noncanonical Nucleic Acid Structures in Their Lifecyclesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-163434en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:36en
sync.item.modts2025.01.17 18:45:07en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
thesis.grantorVysoké učení technické v Brně. . Akademie věd ČRcs
thesis.grantorVysoké učení technické v Brně. . Ostravská univerzita v Ostravěcs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
fmicb1101583.pdf
Size:
6.45 MB
Format:
Adobe Portable Document Format
Description:
fmicb1101583.pdf
Download
Collections
Ústav chemie potravin a biotechnologií