Calcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiation

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dc.contributor.authorBlahnová, Veronikacs
dc.contributor.authorVojtová, Lucycs
dc.contributor.authorPavliňáková, Veronikacs
dc.contributor.authorMuchová, Johanacs
dc.contributor.authorFilova, Evacs
dc.coverage.issue8cs
dc.coverage.volume23cs
dc.date.accessioned2022-07-22T14:52:18Z
dc.date.available2022-07-22T14:52:18Z
dc.date.issued2022-04-01cs
dc.description.abstractCollagen I-based foams were modified with calcined or noncalcined hydroxyapatite or calcium phosphates with various particle sizes and pores to monitor their effect on cell interactions. The resulting scaffolds thus differed in grain size, changing from nanoscale to microscopic, and possessed diverse morphological characteristics and resorbability. The materials' biological action was shown on human bone marrow MSCs. Scaffold morphology was identified by SEM. Using viability test, qPCR, and immunohistochemical staining, we evaluated the biological activity of all of the materials. This study revealed that the most suitable scaffold composition for osteogenesis induction is collagen I foam with calcined hydroxyapatite with a pore size of 360 +/- 130 mu m and mean particle size of 0.130 mu m. The expression of osteogenic markers RunX2 and ColI mRNA was promoted, and a strong synthesis of extracellular protein osteocalcin was observed. ColI/calcined HAP scaffold showed significant osteogenic potential, and can be easily manipulated and tailored to the defect size, which gives it great potential for bone tissue engineering applications.en
dc.formattextcs
dc.format.extent1-17cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationInternational Journal of Molecular Sciences. 2022, vol. 23, issue 8, p. 1-17.en
dc.identifier.doi10.3390/ijms23084236cs
dc.identifier.issn1422-0067cs
dc.identifier.other178551cs
dc.identifier.urihttp://hdl.handle.net/11012/208195
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofInternational Journal of Molecular Sciencescs
dc.relation.urihttps://www.mdpi.com/1422-0067/23/8/4236cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1422-0067/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcollagenen
dc.subjectbioceramicsen
dc.subjectosteogenesisen
dc.titleCalcined Hydroxyapatite with Collagen I Foam Promotes Human MSC Osteogenic Differentiationen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-178551en
sync.item.dbtypeVAVen
sync.item.insts2022.11.02 12:52:32en
sync.item.modts2022.11.02 12:14:45en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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