Characterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four EU countries

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dc.contributor.authorGeržová, Lenkacs
dc.contributor.authorBabák, Vladimírcs
dc.contributor.authorSedlář, Karelcs
dc.contributor.authorFaldynová, Marcelacs
dc.contributor.authorVídeňská, Petracs
dc.contributor.authorČejková, Darinacs
dc.contributor.authorNygaard Jensen, Annettecs
dc.contributor.authorDenis, Martinecs
dc.contributor.authorKerouanton, Annaellecs
dc.contributor.authorRicci, Antoniacs
dc.contributor.authorCibin, Veronicacs
dc.contributor.authorÖsterberg, Juliacs
dc.contributor.authorRychlík, Ivancs
dc.coverage.issue7cs
dc.coverage.volume10cs
dc.date.accessioned2020-08-04T11:00:17Z
dc.date.available2020-08-04T11:00:17Z
dc.date.issued2015-06-28cs
dc.description.abstractOne of the recent trends in animal production is the re-appearance of organic farming. The interest of wide public in organic farming is affected by interest in animal welfare and lower use of antibiotics in organic than in conventional farming systems. On the other hand, the ambition to provide the animals with a more natural life implies their increased exposure to environmental sources of different microorganisms including pathogens. To address these concerns, we determined the abundance of antibiotic resistance and diversity in fecal microbiota in slaughtered pigs kept under conventional and organic farming systems in Sweden, Denmark, France and Italy. Abundance of sul1, sul2, strA, tet(A), tet(B) and cat antibiotic resistance genes was determined by real time PCR genes in 468 samples and the faecal microbiota diversity was characterised in selected 48 samples by pyrosequencing of V3/V4 regions of 16S rRNA. Unlike expectation, there were no significant differences between the abundance of tested antibiotic resistance genes in microbiota of organic and conventional pigs within the same country. There were also no differences in microbiota composition of organic and conventional pigs. The only significant difference was the difference in the abundance of antibiotic resistance genes in the samples from different countries. Fecal microbiota in the samples originating from southern countries (Italy, France) exhibited significantly higher antibiotic resistance gene abundance than those from northern parts of Europe (Denmark, Sweden). Concerning the antibiotic resistance in fecal microbiota, the effect of geographic location of a herd therefore dominates over its organic or conventional production system status.en
dc.description.abstractJedním z nedávných trendů ve zvířecí produkci je znovuobjevení organického hospodářství. Zájem veřejnosti budí predevším lepší prostřední pro zvířata a méně používaná antibiotika. V této práci se zabýváme analýzou genů antibiotické rezistence ve střevní miktoflóře prasat, jak z organických, tak konvenčních farem.cs
dc.formattextcs
dc.format.extent1-10cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLOS ONE. 2015, vol. 10, issue 7, p. 1-10.en
dc.identifier.doi10.1371/journal.pone.0132892cs
dc.identifier.issn1932-6203cs
dc.identifier.other115108cs
dc.identifier.urihttp://hdl.handle.net/11012/69204
dc.language.isoencs
dc.publisherPloscs
dc.relation.ispartofPLOS ONEcs
dc.relation.urihttp://europepmc.org/articles/PMC4517930?pdf=rendercs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1932-6203/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectsložení mikroflóry
dc.subject16S rRNA
dc.subjectantibiotická rezistence
dc.subjectmicrobiota compositionen
dc.subject16S rRNAen
dc.subjectantibiotic rezistanceen
dc.titleCharacterization of antibiotic resistance gene abundance and microbiota composition in feces of organic and conventional pigs from four EU countriesen
dc.title.alternativeCharakterizace složení mikroflóry a zastoupení genů antibiotické rezistence ve výkalech prasat z organických a konvenčních farem čtyř zemí Evropské uniecs
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-115108en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:00:17en
sync.item.modts2020.08.04 12:18:58en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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