Assessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopy

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dc.contributor.authorDzurendová, Simonacs
dc.contributor.authorShapaval, Volhacs
dc.contributor.authorTafintseva, Valeriacs
dc.contributor.authorKohler, Achimcs
dc.contributor.authorByrtusová, Danacs
dc.contributor.authorSzotkowski, Martincs
dc.contributor.authorMárová, Ivanacs
dc.contributor.authorZimmermann, Boriscs
dc.coverage.issue13cs
dc.coverage.volume22cs
dc.date.issued2021-06-23cs
dc.description.abstractOleaginous filamentous fungi can accumulate large amount of cellular lipids and biopolymers and pigments and potentially serve as a major source of biochemicals for food, feed, chemical, pharmaceutical, and transport industries. We assessed suitability of Fourier transform (FT) Raman spectroscopy for screening and process monitoring of filamentous fungi in biotechnology. Six Mucoromycota strains were cultivated in microbioreactors under six growth conditions (three phosphate concentrations in the presence and absence of calcium). FT-Raman and FT-infrared (FTIR) spectroscopic data was assessed in respect to reference analyses of lipids, phosphorus, and carotenoids by using principal component analysis (PCA), multiblock or consensus PCA, partial least square regression (PLSR), and analysis of spectral variation due to different design factors by an ANOVA model. All main chemical biomass constituents were detected by FT-Raman spectroscopy, including lipids, proteins, cell wall carbohydrates, and polyphosphates, and carotenoids. FT-Raman spectra clearly show the effect of growth conditions on fungal biomass. PLSR models with high coefficients of determination (0.83-0.94) and low error (approximately 8%) for quantitative determination of total lipids, phosphates, and carotenoids were established. FT-Raman spectroscopy showed great potential for chemical analysis of biomass of oleaginous filamentous fungi. The study demonstrates that FT-Raman and FTIR spectroscopies provide complementary information on main fungal biomass constituents.en
dc.formattextcs
dc.format.extent1-23cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2021, vol. 22, issue 13, p. 1-23.en
dc.identifier.doi10.3390/ijms22136710cs
dc.identifier.issn2076-2607cs
dc.identifier.orcid0000-0001-8286-2349cs
dc.identifier.orcid0000-0002-2095-1837cs
dc.identifier.other177294cs
dc.identifier.researcheridA-6897-2016cs
dc.identifier.scopus6603465110cs
dc.identifier.urihttp://hdl.handle.net/11012/204970
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/1422-0067/22/13/6710cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectoleaginous microorganismsen
dc.subjectbiodieselen
dc.subjectpigmentsen
dc.subjectbiopolymersen
dc.subjectcarotenoidsen
dc.subjectfatty acidsen
dc.subjectchitinen
dc.subjectchitosanen
dc.subjectfermentationen
dc.subjectfungien
dc.titleAssessment of Biotechnologically Important Filamentous Fungal Biomass by Fourier Transform Raman Spectroscopyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-177294en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:41en
sync.item.modts2025.01.17 15:17:44en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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