Degradation of P(3HB-co-4HB) Films in Simulated Body Fluids

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dc.contributor.authorVodička, Jurajcs
dc.contributor.authorWikarská, Monikacs
dc.contributor.authorTrudičová, Monikacs
dc.contributor.authorJuglová, Zuzanacs
dc.contributor.authorPospíšilová, Anetacs
dc.contributor.authorKalina, Michalcs
dc.contributor.authorSlaninová, Evacs
dc.contributor.authorObruča, Stanislavcs
dc.contributor.authorSedláček, Petrcs
dc.coverage.issue10cs
dc.coverage.volume14cs
dc.date.accessioned2022-05-26T14:52:07Z
dc.date.available2022-05-26T14:52:07Z
dc.date.issued2022-05-13cs
dc.description.abstractA novel model of biodegradable PHA copolymer films preparation was applied to evaluate the biodegradability of various PHA copolymers and to discuss its biomedical applicability. In this study, we illustrate the potential biomaterial degradation rate affectability by manipulation of monomer composition via controlling the biosynthetic strategies. Within the experimental investigation, we have prepared two different copolymers of 3-hydroxybutyrate and 4-hydroxybutyrate-P(3HB-co-36 mol.% 4HB) and P(3HB-co-66 mol.% 4HB), by cultivating the thermophilic bacterial strain Aneurinibacillus sp. H1 and further investigated its degradability in simulated body fluids (SBFs). Both copolymers revealed faster weight reduction in synthetic gastric juice (SGJ) and artificial colonic fluid (ACF) than simple homopolymer P3HB. In addition, degradation mechanisms differed across tested polymers, according to SEM micrographs. While incubated in SGJ, samples were fragmented due to fast hydrolysis sourcing from substantially low pH, which suggest abiotic degradation as the major degradation mechanism. On the contrary, ACF incubation indicated obvious enzymatic hydrolysis. Further, no cytotoxicity of the waste fluids was observed on CaCO-2 cell line. Based on these results in combination with high production flexibility, we suggest P(3HB-co-4HB) copolymers produced by Aneurinibacillus sp. H1 as being very auspicious polymers for intestinal in vivo treatments.en
dc.formattextcs
dc.format.extent1-14cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPolymers. 2022, vol. 14, issue 10, p. 1-14.en
dc.identifier.doi10.3390/polym14101990cs
dc.identifier.issn2073-4360cs
dc.identifier.other177660cs
dc.identifier.urihttp://hdl.handle.net/11012/204434
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofPolymerscs
dc.relation.urihttps://www.mdpi.com/2073-4360/14/10/1990cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2073-4360/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectpolyhydroxyalkanoates (PHA)en
dc.subjectAneurinibacillus sp. H1en
dc.subjectPHA copolymersen
dc.subjectbiodegradationen
dc.subjectsimulated body fluidsen
dc.titleDegradation of P(3HB-co-4HB) Films in Simulated Body Fluidsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-177660en
sync.item.dbtypeVAVen
sync.item.insts2023.02.24 12:52:27en
sync.item.modts2023.02.24 12:14:29en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Fakulta chemickács
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