Addressing cancer invasion and cell motility with quantitative light microscopy

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dc.contributor.authorZicha, Danielcs
dc.coverage.issue1cs
dc.coverage.volume12cs
dc.date.issued2022-01-10cs
dc.description.abstractThe incidence of death caused by cancer has been increasing worldwide. The growth of cancer cells is not the main problem. The majority of deaths are due to invasion and metastasis, where cancer cells actively spread from primary tumors. Our inbred rat model of spontaneous metastasis revealed dynamic phenotype changes in vitro correlating with the metastatic potential in vivo and led to a discovery of a metastasis suppressor, protein 4.1B, which affects their 2D motility on flat substrates. Subsequently, others confirmed 4.1B as metastasis suppressor using knock-out mice and patient data suggesting mechanism involving apoptosis. There is evidence that 2D motility may be differentially controlled to the 3D situation. Here we show that 4.1B affects cell motility in an invasion assay similarly to the 2D system, further supporting our original hypothesis that the role of 4.1B as metastasis suppressor is primarily mediated by its effect on motility. This is encouraging for the validity of the 2D analysis, and we propose Quantitative Phase Imaging with incoherent light source for rapid and accurate testing of cancer cell motility and growth to be of interest for personalized cancer treatment as illustrated in experiments measuring responses of human adenocarcinoma cells to selected chemotherapeutic drugs.en
dc.formattextcs
dc.format.extent1621-1621cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationScientific Reports. 2022, vol. 12, issue 1, p. 1621-1621.en
dc.identifier.doi10.1038/s41598-022-05307-7cs
dc.identifier.issn2045-2322cs
dc.identifier.orcid0000-0002-8808-8064cs
dc.identifier.other178800cs
dc.identifier.researcheridH-9310-2016cs
dc.identifier.scopus55882915900cs
dc.identifier.urihttp://hdl.handle.net/11012/208734
dc.language.isoencs
dc.publisherNATURE PORTFOLIOcs
dc.relation.ispartofScientific Reportscs
dc.relation.urihttps://www.nature.com/articles/s41598-022-05307-7cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2045-2322/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectQuantitative phase imagingen
dc.subjectcancer cell behavioren
dc.titleAddressing cancer invasion and cell motility with quantitative light microscopyen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-178800en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:47:39en
sync.item.modts2025.01.17 15:28:23en
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. ÚFI-odbor optiky a přesné mechanikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Experimentální biofotonikacs
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ÚFI-odbor optiky a přesné mechaniky