Specific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticles

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dc.contributor.authorMura, Monicacs
dc.contributor.authorCarucci, Cristinacs
dc.contributor.authorCaddeo, Elenacs
dc.contributor.authorBačovská, Šárkacs
dc.contributor.authorPiludu, Marcocs
dc.contributor.authorPekař, Miloslavcs
dc.contributor.authorJachimska, Barbaracs
dc.contributor.authorParsons, Drew Franciscs
dc.contributor.authorSalis, Andreacs
dc.coverage.issue1cs
dc.coverage.volume677cs
dc.date.accessioned2025-03-26T11:43:14Z
dc.date.available2025-03-26T11:43:14Z
dc.date.issued2024-08-05cs
dc.description.abstractThe effect of buffer species on biomolecules and biomolecule-nanoparticle interactions is a phenomenon that has been either neglected, or not understood. Here, we study the formation of a BSA protein corona (PC) around amino-functionalized mesoporous silica nanoparticles (MSN-NH2) 2 ) in the presence of different buffers (Tris, BES, cacodylate, phosphate, and citrate) at the same pH (7.15) and different concentrations (10, 50, and 100 mM). We find that BSA adsorption is buffer specific, with the adsorbed amount of BSA being 4.4 times higher in the presence of 100 mM Tris (184 +/- 3 mg/g) than for 100 mM citrate (42 +/- 2 mg/g). That is a considerable difference that cannot be explained by conventional theories. The results become clearer if the interaction energies between BSA and MSN-NH2, 2 , considering the electric double layer (EEDL) EDL ) and the van der Waals (EvdW) vdW ) terms, are evaluated. The buffer specific PC derives from buffer specific zeta potentials that, for MSN-NH2, are positive with Tris and negative with citrate buffers. A reversed sign of zeta potentials can be obtained by considering polarizability-dependent dispersion forces acting together with electrostatics to give the buffer specific outcome. These results are relevant not only to our understanding of the formation of the PC but may also apply to other bio- and nanosystems in biological media.en
dc.formattextcs
dc.format.extent540-547cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationJournal of Colloid and Interface Science. 2024, vol. 677, issue 1, p. 540-547.en
dc.identifier.doi10.1016/j.jcis.2024.07.258cs
dc.identifier.issn0021-9797cs
dc.identifier.orcid0000-0001-9736-9338cs
dc.identifier.orcid0000-0002-3878-0917cs
dc.identifier.other189337cs
dc.identifier.urihttps://hdl.handle.net/11012/250193
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofJournal of Colloid and Interface Sciencecs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0021979724017727cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0021-9797/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectbufferen
dc.subjectprotein coronaen
dc.subjectsilica nanoparticlesen
dc.titleSpecific buffer effects on the formation of BSA protein corona around amino-functionalized mesoporous silica nanoparticlesen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-189337en
sync.item.dbtypeVAVen
sync.item.insts2025.03.26 12:43:14en
sync.item.modts2025.03.26 10:32:29en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
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