The histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasis

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dc.contributor.authorMachelová, Adélacs
dc.contributor.authorDadejová, Martinacs
dc.contributor.authorFranek, Michalcs
dc.contributor.authorMougeot, Guillaumecs
dc.contributor.authorSimon, Laurianecs
dc.contributor.authorLe Goff, Samuelcs
dc.contributor.authorDuc, Celinecs
dc.contributor.authorBassler, Jasmincs
dc.contributor.authorDemko, Martincs
dc.contributor.authorSchwarzerová, Janacs
dc.contributor.authorDesset, Sophiacs
dc.contributor.authorProbst, Alinecs
dc.contributor.authorDvořáčková, Martinacs
dc.coverage.issue3cs
dc.coverage.volume120cs
dc.date.accessioned2025-04-04T11:56:22Z
dc.date.available2025-04-04T11:56:22Z
dc.date.issued2024-10-14cs
dc.description.abstractGenome stability is significantly influenced by the precise coordination of chromatin complexes that facilitate the loading and eviction of histones from chromatin during replication, transcription, and DNA repair processes. In this study, we investigate the role of the Arabidopsis H3 histone chaperones ANTI-SILENCING FUNCTION 1 (ASF1) and HISTONE REGULATOR A (HIRA) in the maintenance of telomeres and 45S rDNA loci, genomic sites that are particularly susceptible to changes in the chromatin structure. We find that both ASF1 and HIRA are essential for telomere length regulation, as telomeres are significantly shorter in asf1a1b and hira mutants. However, these shorter telomeres remain localized around the nucleolus and exhibit a comparable relative H3 occupancy to the wild type. In addition to regulating telomere length, ASF1 and HIRA contribute to silencing 45S rRNA genes and affect their copy number. Besides, ASF1 supports global heterochromatin maintenance. Our findings also indicate that ASF1 transiently binds to the TELOMERE REPEAT BINDING 1 protein and the N terminus of telomerase in vivo, suggesting a physical link between the ASF1 histone chaperone and the telomere maintenance machinery.en
dc.formattextcs
dc.format.extent1125-1141cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationPLANT JOURNAL. 2024, vol. 120, issue 3, p. 1125-1141.en
dc.identifier.doi10.1111/tpj.17041cs
dc.identifier.issn0960-7412cs
dc.identifier.orcid0000-0003-2918-9313cs
dc.identifier.other189692cs
dc.identifier.urihttps://hdl.handle.net/11012/250729
dc.language.isoencs
dc.publisherWileycs
dc.relation.ispartofPLANT JOURNALcs
dc.relation.urihttps://onlinelibrary.wiley.com/doi/abs/10.1111/tpj.17041cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0960-7412/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectFlavobacterium psychrophilumen
dc.subjectGenetic diversityen
dc.subjectAntimicrobial resistanceen
dc.titleThe histone chaperones ASF1 and HIRA are required for telomere length and 45S rDNA copy number homeostasisen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-189692en
sync.item.dbtypeVAVen
sync.item.insts2025.04.04 13:56:22en
sync.item.modts2025.04.03 15:32:05en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
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