The role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineering

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dc.contributor.authorVerčimáková, Katarínacs
dc.contributor.authorKarbowniczek, Joannacs
dc.contributor.authorSedlář, Mariancs
dc.contributor.authorStachewicz, Urszulacs
dc.contributor.authorVojtová, Lucycs
dc.coverage.issue1cs
dc.coverage.volume275cs
dc.date.accessioned2025-02-03T14:51:02Z
dc.date.available2025-02-03T14:51:02Z
dc.date.issued2024-07-02cs
dc.description.abstractVarious strategies have extensively explored enhancing the physical and biological properties of chitosan and cellulose scaffolds for skin tissue engineering. This study presents a straightforward method involving the addition of glycerol into highly porous structures of two polysaccharide complexes: chitosan/carboxymethyl cellulose (Chit/CMC) and chitosan/oxidized cellulose (Chit/OC); during a one-step freeze-drying process. Adding glycerol, especially to Chit/CMC, significantly increased stability, prevented degradation, and improved mechanical strength by nearly 50%. Importantly, after 21 days of incubation in enzymatic medium Chit/CMC scaffold has almost completely decomposed, while foams reinforced with glycerol exhibited only 40% mass loss. It is possible due to differences in multivalent cations and polymer chain contraction, resulting in varied hydrogen bonding and, consequently, distinct physicochemical outcomes. Additionally, the scaffolds with glycerol improved the cellular activities resulting in over 40% higher proliferation of fibroblast after 21 days of incubation. It was achieved by imparting water resistance to the highly absorbent material and aiding in achieving a balance between hydrophilic and hydrophobic properties. This study clearly indicates the possible elimination of additional crosslinkers and multiple fabrication steps that can reduce the cost of scaffold production for skin tissue engineering applications while tailoring mechanical strength and degradation.en
dc.formattextcs
dc.format.extent1-12cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES. 2024, vol. 275, issue 1, p. 1-12.en
dc.identifier.doi10.1016/j.ijbiomac.2024.133602cs
dc.identifier.issn1879-0003cs
dc.identifier.orcid0000-0002-4898-8041cs
dc.identifier.orcid0000-0003-4536-3983cs
dc.identifier.orcid0000-0001-5281-7045cs
dc.identifier.other189828cs
dc.identifier.researcheridD-7762-2012cs
dc.identifier.scopus12039667200cs
dc.identifier.urihttps://hdl.handle.net/11012/250001
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofINTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULEScs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0141813024044076cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1879-0003/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectChitosanen
dc.subjectCelluloseen
dc.subjectIonic complexen
dc.subjectGlycerolen
dc.subjectTissue engineeringen
dc.titleThe role of glycerol in manufacturing freeze-dried chitosan and cellulose foams for mechanically stable scaffolds in skin tissue engineeringen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-189828en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:51:02en
sync.item.modts2025.02.03 09:32:13en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Pokročilé biomateriálycs
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