Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

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dc.contributor.authorSzotkowski, Martincs
dc.contributor.authorByrtusová, Danacs
dc.contributor.authorNěmcová, Andreacs
dc.contributor.authorVysoká, Mariecs
dc.contributor.authorRapta, Marekcs
dc.contributor.authorShapaval, Volhacs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue11cs
dc.coverage.volume7cs
dc.date.issued2019-11-19cs
dc.description.abstractCarotenogenic yeasts are non-conventional oleaginous microorganisms capable to utilize various waste substrates. In this work 4 red yeast strains (Rhodotorula, Cystofilobasidium and Sporobolomyces sp.) were cultivated in media containing crude, emulsified and enzymatically hydrolysed animal waste fat, compared with glucose and glycerol as single C-sources. Cell morphology (cryo-SEM, TEM), production of biomass, lipase, biosurfactants, lipids (GC/FID) carotenoids, ubiquinone, ergosterol (HPLC/PDA) in yeast cells was studied depending on medium composition, C-source and C/N ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N=13 was higher on emulsified/hydrolysed fat than on glucose/glycerol. Production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/l) and lipids (54.5%/CDW) were found in S.pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. Increased C/N ratio (13-100) led to higher biomass production in fat media. Production of total lipids increased in all strains to C/N 50. Oppositely, production of carotenoids, ubiquinone and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts are having great application potential and can result from valorization of animal waste fat in the biorefinery concept.en
dc.description.abstractKarotenogenní kvasinky jsou schopny utilizovat řadu různých substrátů včetně odpadů. V této práci je popsána kultivace vybraných kmenů karotenogenních kvasinek rodů Rhodotorula, Cystofilobasidium, and Sporobolomyces sp. v médiu obsahujícícm surový odpadní živočišný tuk a upravený substrát emulsifikací a enzymovou hydrolýzou. Výsledky byly srovnány s kultivací na glukóze a glycerolu jako jednoduchých zdrojích uhlíku.cs
dc.formattextcs
dc.format.extent1-28cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2019, vol. 7, issue 11, p. 1-28.en
dc.identifier.doi10.3390/microorganisms7110578cs
dc.identifier.issn2076-2607cs
dc.identifier.orcid0000-0002-1164-1837cs
dc.identifier.orcid0000-0002-9297-0703cs
dc.identifier.orcid0000-0002-2095-1837cs
dc.identifier.other160868cs
dc.identifier.researcheridABG-3347-2021cs
dc.identifier.researcheridA-6897-2016cs
dc.identifier.scopus25621457300cs
dc.identifier.scopus6603465110cs
dc.identifier.urihttp://hdl.handle.net/11012/184660
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/2076-2607/7/11/578cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectcarotenogenic yeastsen
dc.subjectlipidsen
dc.subjectcarotenoidsen
dc.subjectlipaseen
dc.subjectbiosurfactantsen
dc.subjectubiquinoneen
dc.subjectergosterolen
dc.titleStudy of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrateen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-160868en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:34en
sync.item.modts2025.01.17 16:55:05en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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