Enzyme-Based Impedimetric Biosensor dotted with gold nanoparticles

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but.event.date23.04.2024cs
but.event.titleSTUDENT EEICT 2024cs
dc.contributor.authorKošelová, Zuzana
dc.contributor.authorFohlerová, Zdenka
dc.date.accessioned2024-07-09T07:47:50Z
dc.date.available2024-07-09T07:47:50Z
dc.date.issued2024cs
dc.description.abstractThis research delves into the realm of biosensor improvement through the utilization of gold nanoparticles (Au NPs). The primary objective is to assess the impact of differentsized Au NPs on sensor performance, specifically investigating whether 100 nm or 20 nm nanoparticles prove more favourable to enhancement. Moreover, we aim to inspect the biosensor's response to varied concentrations of Au NPs, unravelling the involved collaboration between nanoparticle size, concentration, and overall sensor properties. This modification of commercial electrodes with Au NPs, could be way for enhancing surface area and enzyme immobilization. Notably, the investigation also explores the potential drawbacks associated with increasing nanoparticle concentration and offers insights into optimizing biosensor design. It has been observed that while 20 nm Au NPs slightly decreased impedance values at higher glucose concentrations, 100 nm Au NPs, conversely, exhibited an increase in capacitive behaviour. Equally crucial is the parameter chosen for constructing the calibration curve. From impedance values at low frequencies of alternating voltage, such as 2 Hz, a lower Limit of Detection (LOD) is obtained. However, the analysis of Rct in the case of 20 nm Au NPs reveals a broader range of glucose concentrations falling within the calibration area. Through a comprehensive analysis of electrochemical behaviour, impedance, and charge transfer resistance, we endeavour to provide contributions to the improvement of biosensor technologies.en
dc.formattextcs
dc.format.extent202-206cs
dc.format.mimetypeapplication/pdfen
dc.identifier.citationProceedings II of the 30st Conference STUDENT EEICT 2024: Selected papers. s. 202-206. ISBN 978-80-214-6230-4cs
dc.identifier.doi10.13164/eeict.2024.202
dc.identifier.isbn978-80-214-6230-4
dc.identifier.issn2788-1334
dc.identifier.urihttps://hdl.handle.net/11012/249315
dc.language.isoencs
dc.publisherVysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.relation.ispartofProceedings II of the 30st Conference STUDENT EEICT 2024: Selected papersen
dc.relation.urihttps://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_2.pdfcs
dc.rights© Vysoké učení technické v Brně, Fakulta elektrotechniky a komunikačních technologiícs
dc.rights.accessopenAccessen
dc.subjectgold nanoparticlesen
dc.subjectbiosensorsen
dc.subjectenzyme immobilizationen
dc.subjectimpedance spectroscopyen
dc.subjectglucose detectionen
dc.subjectcalibration curvesen
dc.titleEnzyme-Based Impedimetric Biosensor dotted with gold nanoparticlesen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
eprints.affiliatedInstitution.departmentFakulta elektrotechniky a komunikačních technologiícs
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