Nebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systems

Simple item page
dc.contributor.authorMišík, Ondrejcs
dc.contributor.authorSzabová, Janacs
dc.contributor.authorCejpek, Ondřejcs
dc.contributor.authorMalý, Milancs
dc.contributor.authorJugl, Adamcs
dc.contributor.authorBělka, Miloslavcs
dc.contributor.authorMravec, Filipcs
dc.contributor.authorLízal, Františekcs
dc.coverage.issue4cs
dc.coverage.volume21cs
dc.date.issued2024-03-11cs
dc.description.abstractLiposomal carrier systems have emerged as a promising technology for pulmonary drug delivery. This study focuses on two selected liposomal systems, namely, dipalmitoylphosphatidylcholine stabilized by phosphatidic acid and cholesterol (DPPC-PA-Chol) and dipalmitoylphosphatidylcholine stabilized by polyethylene glycol and cholesterol (DPPC-PEG-Chol). First, the research investigates the stability of these liposomal systems during the atomization process using different kinds of nebulizers (air-jet, vibrating mesh, and ultrasonic). The study further explores the aerodynamic particle size distribution of the aerosol generated by the nebulizers. The nebulizer that demonstrated optimal stability and particle size was selected for more detailed investigation, including Andersen cascade impactor measurements, an assessment of the influence of flow rate and breathing profiles on aerosol particle size, and an in vitro deposition study on a realistic replica of the upper airways. The most suitable combination of a nebulizer and liposomal system was DPPC-PA-Chol nebulized by a Pari LC Sprint Star in terms of stability and particle size. The influence of the inspiration flow rate on the particle size was not very strong but was not negligible either (decrease of D-v50 by 1.34 mu m with the flow rate increase from 8 to 60 L/min). A similar effect was observed for realistic transient inhalation. According to the in vitro deposition measurement, approximately 90% and 70% of the aerosol penetrated downstream of the trachea using the stationary flow rate and the realistic breathing profile, respectively. These data provide an image of the potential applicability of liposomal carrier systems for nebulizer therapy. Regional lung drug deposition is patient-specific; therefore, deposition results might vary for different airway geometries. However, deposition measurement with realistic boundary conditions (airway geometry, breathing profile) brings a more realistic image of the drug delivery by the selected technology. Our results show how much data from cascade impactor testing or estimates from the fine fraction concept differ from those of a more realistic case.en
dc.formattextcs
dc.format.extent1848-1860cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMOLECULAR PHARMACEUTICS. 2024, vol. 21, issue 4, p. 1848-1860.en
dc.identifier.doi10.1021/acs.molpharmaceut.3c01146cs
dc.identifier.issn1543-8384cs
dc.identifier.orcid0000-0003-4683-5145cs
dc.identifier.orcid0000-0001-7638-8759cs
dc.identifier.orcid0000-0002-7854-9533cs
dc.identifier.orcid0000-0002-1193-519Xcs
dc.identifier.orcid0000-0003-0602-2334cs
dc.identifier.orcid0000-0001-8905-9751cs
dc.identifier.orcid0000-0001-6114-4751cs
dc.identifier.orcid0000-0002-0389-608Xcs
dc.identifier.other188404cs
dc.identifier.researcheridAAY-7288-2021cs
dc.identifier.researcheridG-7170-2015cs
dc.identifier.researcheridD-4363-2018cs
dc.identifier.scopus57189715785cs
dc.identifier.scopus12039611100cs
dc.identifier.scopus54966786300cs
dc.identifier.urihttp://hdl.handle.net/11012/245516
dc.language.isoencs
dc.publisherAmerican Chemical Societycs
dc.relation.ispartofMOLECULAR PHARMACEUTICScs
dc.relation.urihttps://pubs.acs.org/doi/10.1021/acs.molpharmaceut.3c01146cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1543-8384/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectliposomeen
dc.subjectaerosolen
dc.subjectparticle sizeen
dc.subjectnebulizeren
dc.subjectpulmonary drug deliveryen
dc.subjectinhalationen
dc.subjectdepositionen
dc.titleNebulization and In Vitro Upper Airway Deposition of Liposomal Carrier Systemsen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-188404en
sync.item.dbtypeVAVen
sync.item.insts2024.06.17 09:47:03en
sync.item.modts2024.06.17 09:12:52en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav fyzikální a spotřební chemiecs
thesis.grantorVysoké učení technické v Brně. Fakulta strojního inženýrství. Energetický ústavcs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
misiketal2024nebulizationandinvitroupperairwaydepositionofliposomalcarriersystems.pdf
Size:
5.26 MB
Format:
Adobe Portable Document Format
Description:
file misiketal2024nebulizationandinvitroupperairwaydepositionofliposomalcarriersystems.pdf
Download
Collections
Ústav fyzikální a spotřební chemie
Energetický ústav