Bioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast Rhodotorula kratochvilovae and Several Microalgae under Stress

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dc.contributor.authorSzotkowski, Martincs
dc.contributor.authorHolub, Jiřícs
dc.contributor.authorŠimanský, Samuelcs
dc.contributor.authorHubačová, Kláracs
dc.contributor.authorSikorová, Pavlínacs
dc.contributor.authorMariničová, Veronikacs
dc.contributor.authorNěmcová, Andreacs
dc.contributor.authorMárová, Ivanacs
dc.coverage.issue6cs
dc.coverage.volume9cs
dc.date.issued2021-05-28cs
dc.description.abstractThe co-cultivation of red yeasts and microalgae works with the idea of the natural transport of gases. The microalgae produce oxygen, which stimulates yeast growth, while CO2 produced by yeast is beneficial for algae growth. Both microorganisms can then produce lipids. The present pilot study aimed to evaluate the ability of selected microalgae and carotenogenic yeast strains to grow and metabolize in co-culture. The effect of media composition on growth and metabolic activity of red yeast strains was assessed simultaneously with microalgae mixotrophy. Cultivation was transferred from small-scale co-cultivation in Erlenmeyer flasks to aerated bottles with different inoculation ratios and, finally, to a 3L bioreactor. Among red yeasts, the strain R. kratochvilovae CCY 20-2-26 was selected because of the highest biomass production on BBM medium. Glycerol is a more suitable carbon source in the BBM medium and urea was proposed as a compromise. From the tested microalgae, Desmodesmus sp. were found as the most suitable for co-cultivations with R. kratochvilovae. In all co-cultures, linear biomass growth was found (144 h), and the yield was in the range of 8.78-11.12 g/L of dry biomass. Lipids increased to a final value of 29.62-31.61%. The FA profile was quite stable with the UFA portion at about 80%. Around 1.98-2.49 mg/g CDW of carotenoids with torularhodine as the major pigment were produced, ubiquinone production reached 5.41-6.09 mg/g, and ergosterol yield was 6.69 mg/g. Chlorophyll production was very low at 2.11 mg/g. Pilot experiments have confirmed that carotenogenic yeasts and microalgae are capable of symbiotic co-existence with a positive impact om biomass growth and lipid metabolites yields.en
dc.formattextcs
dc.format.extent1-23cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMicroorganisms. 2021, vol. 9, issue 6, p. 1-23.en
dc.identifier.doi10.3390/microorganisms9061160cs
dc.identifier.issn2076-2607cs
dc.identifier.orcid0000-0002-0982-5696cs
dc.identifier.orcid0000-0002-1164-1837cs
dc.identifier.orcid0000-0002-2095-1837cs
dc.identifier.other172475cs
dc.identifier.researcheridABG-3347-2021cs
dc.identifier.researcheridA-6897-2016cs
dc.identifier.scopus25621457300cs
dc.identifier.scopus6603465110cs
dc.identifier.urihttp://hdl.handle.net/11012/201639
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofMicroorganismscs
dc.relation.urihttps://www.mdpi.com/2076-2607/9/6/1160cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2076-2607/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectco-cultivationen
dc.subjectcarotenogenic yeastsen
dc.subjectcarotenoidsen
dc.subjectDesmodesmus spen
dc.subjectmicroalgaeen
dc.subjectlipidsen
dc.subjectRhodotorula kratochvilovaeen
dc.titleBioreactor Co-Cultivation of High Lipid and Carotenoid Producing Yeast Rhodotorula kratochvilovae and Several Microalgae under Stressen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172475en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:38:39en
sync.item.modts2025.01.17 18:36:35en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie potravin a biotechnologiícs
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