Genosensing on a 3D-printed nanocarbon electrode

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dc.contributor.authorJyoti, Jyotics
dc.contributor.authorFojta, Miroslavcs
dc.contributor.authorHermanová, Monikacs
dc.contributor.authorPivoňková, Hanacs
dc.contributor.authorAlduhaish, Osamahcs
dc.contributor.authorPumera, Martincs
dc.coverage.issue1cs
dc.coverage.volume151cs
dc.date.issued2023-06-01cs
dc.description.abstractIn this paper we present the characterization of 3D-printed nanocarbon electrodes (3DnCes) and their application in electrochemical enzyme-linked detection of DNA hybridization. The approach takes advantage of a facile procedure based on adsorption of target DNA on the electrode surface followed by hybridization with a biotinylated probe and binding of streptavidin-alkaline phosphatase conjugate. The alkaline phosphatase converts 1-naphthyl phosphate in the background electrolyte into electrochemically oxidizable 1-naphthol, which is subsequently detected using linear sweep voltammetry. The preparation, characterization, and analytical performance of the 3DnCes are reported. The results show the applicability of such 3DnCes in detection of target DNA hybridization specifically with the complementary biotinylated probe, and indicate the potential of 3D printed electrodes for use in various bioanalytical approaches.en
dc.formattextcs
dc.format.extent8cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationELECTROCHEMISTRY COMMUNICATIONS. 2023, vol. 151, issue 1, 8 p.en
dc.identifier.doi10.1016/j.elecom.2023.107508cs
dc.identifier.issn1873-1902cs
dc.identifier.orcid0000-0001-5846-2951cs
dc.identifier.other186966cs
dc.identifier.researcheridF-2724-2010cs
dc.identifier.urihttp://hdl.handle.net/11012/244975
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofELECTROCHEMISTRY COMMUNICATIONScs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S1388248123000826cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1873-1902/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subject3D-printed nanocarbon electrodeen
dc.subjectDNA hybridizationen
dc.subjectElectrochemical analysisen
dc.subjectAdditive manufacturingen
dc.titleGenosensing on a 3D-printed nanocarbon electrodeen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-186966en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:50:34en
sync.item.modts2025.01.17 16:50:03en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Energie budoucnosti a inovacecs
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