Imaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM.

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dc.contributor.authorHřib, Jiřícs
dc.contributor.authorVooková, Boženacs
dc.contributor.authorNeděla, Vilémcs
dc.coverage.issue1cs
dc.coverage.volume10cs
dc.date.accessioned2021-10-01T10:53:38Z
dc.date.available2021-10-01T10:53:38Z
dc.date.issued2015-01-01cs
dc.description.abstractEnvironmental scanning electron microscopy enables the investigation of uncoated pine early embryogenic tissue samples in situ. The samples were examined under low vacuum conditions (air pressure 550 Pa) at a temperature of around -18°C by the AQUASEM II noncommercial environmental scanning electron microscope. The native extracellular matrix surface network was imaged by the environmental scanning electron microscope and in dark field mode of the optical microscope too. The backscattered electron detector disclosed brightness loci in the cells of early embryogenic culture. This work shows images of native pine embryogenic tissues. The continuity of extracellular matrix with structural integrity of plant organism is discussed.en
dc.formattextcs
dc.format.extent285-290cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationOpen Life Sciences. 2015, vol. 10, issue 1, p. 285-290.en
dc.identifier.doi10.1515/biol-2015-0028cs
dc.identifier.issn2391-5412cs
dc.identifier.other158803cs
dc.identifier.urihttp://hdl.handle.net/11012/201715
dc.language.isoencs
dc.publisherDe Gruyter Open Ltdcs
dc.relation.ispartofOpen Life Sciencescs
dc.relation.urihttp://www.degruyter.com/view/j/biol.2015.10.issue-1/biol-2015-0028/biol-2015-0028.xml?format=INTcs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2391-5412/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectsomatic embryogenesis * backscattered electron detector * ionization detector * bottle cells * extracellular matrix * structural integrityen
dc.titleImaging of native early embryogenic tissue of Scots pine (Pinus sylvestris L.) by ESEM.en
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-158803en
sync.item.dbtypeVAVen
sync.item.insts2021.10.01 12:53:38en
sync.item.modts2021.10.01 12:15:26en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav elektrotechnologiecs
thesis.grantorVysoké učení technické v Brně. . Ústav přístrojové techniky AV ČRcs
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