Kryty ran připravené z nanokompozitního materiálu

Abstract

Boli pripravené kryty rán na bázi prírodných látok polyvinyl alcohol/ hyaluronan/ strieborné nanočastice (PVA/ HA/ Ag-NPs). Hyaluronan bol použitý ako redukčné a stabilizačné činidlo pre syntézu nanočastíc striebra. Pri príprave Ag-NPs boli testované viaceré parametre ako koncentrácia dusičnanu strieborného ako zdroja Ag-NPs (0,01; 0,1;0,5;1 M), koncentrácia kyseliny hyalurónovej (1,2 %) a jej rozdielna molekulová hmotnosť. Kryty rán z nanovlákien boli pripravené pomocou techniky electro-spinning z roztokov líšiacich sa pomerom PVA a HA/Ag-NPs (100; 90/10; 80/20; 60/40; 50/50). Vlastnosti nanokompozitu HA/Ag-NPs boli hodnotené pomocou TEM, reológie, DLS, XRD, UV/Vis spektroskopie a kryty rán boli charakterizované pomocou SEM, TGA, FTIR a ťahovej skúšky.New wound dressing based on nanocomposite polyvinyl alcohol/hyaluronan/ silver nanoparticles (PVA/HA/Ag-NPs) was prepared by green way. Hyaluronan was used as reducing and stabilizing agent for hyaluronan for silver nanoparticle preparation. Different parameters were investigated for preparation of Ag-NPs like concentration of silver nitrate as a source of Ag-NPs (0.01, 0.1, 0.5, 1M), concentration of HA (1, 2 %) and molecular weight of HA (50 kDA, 1,7 MDa). The nanofibers dressing sheet were fabricated by electro-spinning technique using different ratios between PVA and HA/Ag-NPs (100; 90/10; 80/20; 64/40; 50/50). The nanocomposites were evaluated by TEM, rheology, DLS, UV/Vis spectroscope, and the wound dressing nanofibers were characterized by SEM, TGA, FTIR, XRD and the mechanical properties were measured and evaluated.