Elegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detection

Simple item page
dc.contributor.authorAssi, Navidcs
dc.contributor.authorPavelicová, Kristýnacs
dc.contributor.authorVaculovičová, Markétacs
dc.contributor.authorVaculovič, Tomášcs
dc.date.accessioned2021-07-14T14:55:52Z
dc.date.available2021-07-14T14:55:52Z
dc.date.issued2021-07-12cs
dc.description.abstractAntibody labeling with a signal-providing tag (e.g. an enzyme, chromophore, fluorophore, metal chelate, radioisotope, etc.) is a key procedure in bioanalytical techniques such an enzyme-linked immunosorbent assay, western blot, or immunohistochemical analysis. The process may be laborious, impair the activity, and time-consuming. Concisely, in this work, an elegant method of rapid and effective antibody labeling by quantum dots was developed taking advantage of partial reduction of antibody structure and UV-induced quantum dot formation. Zinc and cadmium with the thiol group of the antibody as capping agents stabilizing the fluorescent nanoparticle while the biorecognition capabilities were maintained. The ZnCd/S quantum dots creation has to prosper detected with capillary electrophoresis (CE) and laser ablation ICP- MS (LA- ICP- MS).en
dc.formattextcs
dc.format.extent395-398cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationProceedings 12th International Conference on Nanomaterials - Research & Application. 2021, p. 395-398.en
dc.identifier.doi10.37904/nanocon.2020.3752cs
dc.identifier.isbn978-80-87294-98-7cs
dc.identifier.issn2694-930Xcs
dc.identifier.other172014cs
dc.identifier.urihttp://hdl.handle.net/11012/200499
dc.language.isoencs
dc.publisherTangercs
dc.relation.ispartofProceedings 12th International Conference on Nanomaterials - Research & Applicationcs
dc.relation.urihttps://www.confer.cz/nanocon/2020/3752-elegant-rapid-and-simple-procedure-for-labeling-of-antibodies-with-fluorescent-cdzn-s-nanoparticles-for-la-icp-ms-detectioncs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/2694-930X/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectAntibodyen
dc.subjectantigenen
dc.subjectquantum dotsen
dc.subjectcleavage disulfide bondsen
dc.subjectfluorescenten
dc.titleElegant, rapid and simple procedure for labeling of antibodies with fluorescent ZnCd/S quantum dots for laser ablation ICP-MS detectionen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-172014en
sync.item.dbtypeVAVen
sync.item.insts2021.07.14 16:55:52en
sync.item.modts2021.07.14 16:14:09en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Elegant rapid and simple procedure for labeling of antibodies with fluorescent ZnCd_S quantum dots for laser ablation ICPMS det.pdf
Size:
2.38 MB
Format:
Adobe Portable Document Format
Description:
Elegant rapid and simple procedure for labeling of antibodies with fluorescent ZnCd_S quantum dots for laser ablation ICPMS det.pdf
Download
Collections
Chytré nanonástroje