Isolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oil

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dc.contributor.authorPospíšilová, Anetacs
dc.contributor.authorNováčková, Ivanacs
dc.contributor.authorPřikryl, Radekcs
dc.coverage.issue1cs
dc.coverage.volume326cs
dc.date.accessioned2021-11-30T11:55:15Z
dc.date.available2021-11-30T11:55:15Z
dc.date.issued2021-04-01cs
dc.description.abstractThe aim of this work was to develop a soap-based method for the isolation of poly(3-hydroxybutyrate) from bacterial biomass. The method consisted of adding soap derived from waste cooking oil to a concentrated (25%) biomass suspension, heating and centrifugal separation. Purity above 95% could be achieved with soap:cell dry mass ratios at least 0.125 g/g, making the method comparable to other surfactant-based protocols. Molecular weights M, of products from all experiments were between 350 and 450 kDa, being high enough for future material applications. Addition of hydrochloric acid to the wastewater led to the precipitation of soap and part of non-P3HB cell mass. The resulting precipitate was utilized as a carbon source in biomass production and increased substrate-to-P3HB conversion.en
dc.description.embargo2023-01-17cs
dc.formattextcs
dc.format.extent1-5cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationBIORESOURCE TECHNOLOGY. 2021, vol. 326, issue 1, p. 1-5.en
dc.identifier.doi10.1016/j.biortech.2021.124683cs
dc.identifier.issn0960-8524cs
dc.identifier.other171646cs
dc.identifier.urihttp://hdl.handle.net/11012/203003
dc.language.isoencs
dc.publisherElseviercs
dc.relation.ispartofBIORESOURCE TECHNOLOGYcs
dc.relation.urihttps://www.sciencedirect.com/science/article/pii/S0960852421000213cs
dc.rightsCreative Commons Attribution-NonCommercial-NoDerivatives 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/0960-8524/cs
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/cs
dc.subjectPHAen
dc.subjectP3HBen
dc.subjectRecoveryen
dc.subjectDownstream processen
dc.subjectIsolationen
dc.titleIsolation of poly(3-hydroxybutyrate) from bacterial biomass using soap made of waste cooking oilen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionacceptedVersionen
sync.item.dbidVAV-171646en
sync.item.dbtypeVAVen
sync.item.insts2023.01.17 00:52:51en
sync.item.modts2023.01.17 00:14:35en
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Ústav chemie materiálůcs
thesis.grantorVysoké učení technické v Brně. Fakulta chemická. Centrum materiálového výzkumucs
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