Multi-electrode array with a planar surface for cell patterning by microprinting

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dc.contributor.authorSlavík, Jancs
dc.contributor.authorSkopalík, Josefcs
dc.contributor.authorProvazník, Ivocs
dc.contributor.authorHubálek, Jaromírcs
dc.coverage.issue24cs
dc.coverage.volume19cs
dc.date.accessioned2020-08-04T11:00:35Z
dc.date.available2020-08-04T11:00:35Z
dc.date.issued2019-12-05cs
dc.description.abstractMultielectrode arrays (MEAs) are devices for non-invasive electrophysiological measurements of cell populations. This paper describes a novel fabrication method of MEAs with a fully planar surface. The surface of the insulation layer and the surface of the electrodes were on one plane; we named this device the planar MEA (pMEA). The main advantage of the pMEA is that it allows uniform contact between the pMEA surface and a substrate for positioning of microfluidic channels or microprinting of a cell adhesive layer. The fabrication of the pMEA is based on a low adhesive Au sacrificial peel-off layer. In divergence from conventional MEAs with recessed electrodes, the electrodes of the pMEA lead across the sloped edge of the insulation layer. To make this, the profile of the edge of the insulation layer was measured and the impedance of the planar electrodes was characterized. The impedance of the pMEA was comparable with the impedance of conventional MEA electrodes. The pMEA was tested for patterning HL-1 cells with a combination of imprinting fibronectin and coating by antifouling poly (l-lysine)-graft-poly(ethylene glycol) (PLL-g-PEG). The HL-1 cells remained patterned even at full confluency and presented spontaneous and synchronous beating activity.en
dc.formattextcs
dc.format.extent1-11cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationSENSORS. 2019, vol. 19, issue 24, p. 1-11.en
dc.identifier.doi10.3390/s19245379cs
dc.identifier.issn1424-8220cs
dc.identifier.other161298cs
dc.identifier.urihttp://hdl.handle.net/11012/184124
dc.language.isoencs
dc.publisherMDPIcs
dc.relation.ispartofSENSORScs
dc.relation.urihttps://www.mdpi.com/1424-8220/19/24/5379cs
dc.rightsCreative Commons Attribution 4.0 Internationalcs
dc.rights.accessopenAccesscs
dc.rights.sherpahttp://www.sherpa.ac.uk/romeo/issn/1424-8220/cs
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/cs
dc.subjectmultielectrode arrayen
dc.subjectmicroelectrode arrayen
dc.subjectsacrificial layeren
dc.subjectpeel-offen
dc.subjectHL-1 cellsen
dc.subjectmicroprintingen
dc.subjectantifouling agenten
dc.titleMulti-electrode array with a planar surface for cell patterning by microprintingen
dc.type.driverarticleen
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161298en
sync.item.dbtypeVAVen
sync.item.insts2020.08.04 13:00:35en
sync.item.modts2020.08.04 12:31:45en
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav biomedicínského inženýrstvícs
thesis.grantorVysoké učení technické v Brně. Fakulta elektrotechniky a komunikačních technologií. Ústav mikroelektronikycs
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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