White and black anodic TiO2 nanotubes: Comparison of biological effects in A549 and SH-SY5Y cells

Sopha, Hanna Ingrid; Báčová, Jana; Baishya, Kaushik; Sepúlveda Sepúlveda, Lina Marcela; Rodriguez Pereira, Jhonatan; Čapek, Jan; Hromádko, Luděk; Zazpe Mendioroz, Raúl; Thalluri, Sitaramanjaneya Mouli; Mistrík, Jan; Knotek, Petr; Roušar, Tomáš; Macák, Jan

White and black anodic TiO2 nanotubes: Comparison of biological effects in A549 and SH-SY5Y cells

Files

1s2.0S0257897223002797main.pdf(6.58 MB)

Date

2023-06-15

Authors

Sopha, Hanna Ingrid

Báčová, Jana

Baishya, Kaushik

Sepúlveda Sepúlveda, Lina Marcela

Rodriguez Pereira, Jhonatan

Čapek, Jan

Hromádko, Luděk

Zazpe Mendioroz, Raúl

Thalluri, Sitaramanjaneya Mouli

Mistrík, Jan

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ORCID

0000-0001-6910-8560

0000-0001-7091-3022

Publisher

Elsevier

Altmetrics

Abstract

In this work, the cytotoxicity of black TiO2 nanotubes in comparison to their white counterparts is exploited for the first time. To provide a comprehensive overview, the toxicity is also assessed in comparison to multi-walled carbon nanotubes (MWCNTs) using two different cell lines. The TiO2 nanotube layers were prepared by anodic oxidation of Ti foils and investigated in the as-prepared white form, and in the black form, achieved by reduction via comproportionation reaction of Ti and TiO2. Additionally, a part of the black TiO2 nanotube layers was coated with a thin TiO2 coating with a nominal thickness of similar to 0.275 nm by Atomic Layer Deposition (ALD). Pulmonary A549 cells and neural SH-SY5Y cells were exposed to a wide range of TiO2 nanotube concentrations (1-500 mu g/mL) fo(r) 24 and 48 h. Then, the cytotoxicity was evaluated using cell viability and glutathione assessments. Essentially, no reduction in cell viability was observed after incubating the cells with TiO2 nanotubes up to 100 mu g/mL, while MWCNTs decreased the cell viability at a similar concentration significantly. In addition, ALD TiO2 coating on the black TiO2 nanotubes caused disappearance of mild toxic effects of white and black TiO2 nanotubes due to the shading of carbon and fluorine species incorporated within the TiO2 nanotube walls.

Keywords

TiO2 nanotube layers, Black TiO2, Cell growth, Cytotoxicity, Biocompatibility

Citation

SURFACE & COATINGS TECHNOLOGY. 2023, vol. 462, issue 1, p. 1-11.
https://www.sciencedirect.com/science/article/pii/S0257897223002797

Document type

Peer-reviewed

Document version

Published version

Language of document

en

Document licence

Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
http://creativecommons.org/licenses/by-nc-nd/4.0/