A simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoring

Simple item page
dc.contributor.authorSedláčková, Eliškacs
dc.contributor.authorSmolíková, Vendulacs
dc.contributor.authorBirgusová, Eliškacs
dc.contributor.authorBytešníková, Zuzanacs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorAdam, Vojtěchcs
dc.date.accessioned2020-01-24T11:53:33Z
dc.date.available2020-01-24T11:53:33Z
dc.date.issued2019-12-31cs
dc.description.abstractDNA methylation is one of the well-known epigenetic mechanism which plays a crucial role in the development of various diseases, such as cancer, cardiovascular diseases or diabetes. Electrochemical biosensors promise excellent results for clinical diagnostics, especially in term of their sensitivity, stability, selectivity, portability, they are cost-effective, easy-to-use and provides a fast response. In this study, simply, reliably and selective DNA based electrochemical biosensor for detection of methylated DNA was fabricated. The proposed biosensor was modified with the synthesised reduced graphene oxide combined with gold nanoparticles (rGO-AuNPs). This nanocomposite has shown a strong affinity to the DNA probe and demonstrated promising analytical characteristics. The electrochemical impedance spectroscopy (EIS) was used for the characterization of interface properties of the gold electrode (GE). Additionally, the sensitivity of the developed biosensor was performed by differential pulse voltammetry (DPV) to investigate the activity of enzyme methyltransferase M.SssI (MTase). Fabricated biosensor offers quite a low detection limit (LOD), which was 3.2 U/ml and limit of quantification (LOQ) was 3.3 U/ml.en
dc.formattextcs
dc.format.extent621-626cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 621-626.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.other161397cs
dc.identifier.urihttp://hdl.handle.net/11012/184133
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0120_A-simple-electrochemical-biosensor-for-the-detection-of-methylated-DNA-and-for-methyltransferase-activity-monit.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectelectrochemical biosensoren
dc.subjectmethylated DNAen
dc.subjectreduced graphene oxideen
dc.subjectgold nanoparticlesen
dc.titleA simple electrochemical biosensor for the detection of methylated DNA and for methyltransferase activity monitoringen
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161397en
sync.item.dbtypeVAVen
sync.item.insts2021.03.05 12:54:29en
sync.item.modts2021.03.05 12:14:33en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
A simple electrochemical biosensor for the detection of methylated DNA.pdf
Size:
1.15 MB
Format:
Adobe Portable Document Format
Description:
A simple electrochemical biosensor for the detection of methylated DNA.pdf
Download
Collections
Chytré nanonástroje