Sensitive biosensor for detection oncogenic miRNA-21

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dc.contributor.authorVaňová, Veronikacs
dc.contributor.authorDostálová, Eliškacs
dc.contributor.authorHynek, Davidcs
dc.contributor.authorRichtera, Lukášcs
dc.contributor.authorAdam, Vojtěchcs
dc.date.issued2019-12-31cs
dc.description.abstractThe aim of this study was to prepare a sensitive electrochemical biosensor for detection of oncogenic miRNA-21 as a potential biomarker for early detection of cancer. MicroRNAs (miRNAs) are small noncoding RNA, which play an important role in the regulation of gene expression. miRNAs are present in extracellular fluids like urine, serum, etc. in extremely low concentration. It is important for the biosensor to detect extremely low concentrations of specific miRNA as potential biomarkers. The aim of this experiment was designing, preparing and optimizing a sensitive and selective biosensor for the detection of miRNA-21. A biosensor was prepared to detect the lowest concentration of miRNA-21 in the sample. The linear concentration range for the calibration curve was 1 fM to 10 nM. LOD was 1 fM and obtained from the regression equation was 3.2 zM and LOQ was 10.8 zM. Subsequently, prepared biosensor was measured in artificial urine (AU) samples to verify the functioning of the sensor in induced real conditions. The results showed a minimal effect of the matrix for the determination of the target miRNA.en
dc.formattextcs
dc.format.extent676-681cs
dc.format.mimetypeapplication/pdfcs
dc.identifier.citationMendelNet 2019 Proceedings of 26th International PhD Students Conference. 2019, p. 676-681.en
dc.identifier.isbn978-80-7509-688-3cs
dc.identifier.orcid0000-0003-1367-9414cs
dc.identifier.orcid0000-0002-7318-6470cs
dc.identifier.orcid0000-0002-8288-3999cs
dc.identifier.orcid0000-0002-8527-286Xcs
dc.identifier.other161434cs
dc.identifier.researcheridE-5702-2012cs
dc.identifier.researcheridN-9991-2014cs
dc.identifier.researcheridD-7686-2012cs
dc.identifier.scopus12040049600cs
dc.identifier.urihttp://hdl.handle.net/11012/184137
dc.language.isoencs
dc.publisherMendel University in Brnocs
dc.relation.ispartofMendelNet 2019 Proceedings of 26th International PhD Students Conferencecs
dc.relation.urihttps://mendelnet.cz/artkey/mnt-201901-0130_Sensitive-biosensor-for-detection-oncogenic-miRNA-21.php?back=/magno/mnt/2019/mn1.php?secid=4cs
dc.rights(C) Mendel University in Brnocs
dc.rights.accessopenAccesscs
dc.subjectnucleic acidsen
dc.subjectmiRNAen
dc.subjectelectrochemical detectionen
dc.subjectbiosensoren
dc.titleSensitive biosensor for detection oncogenic miRNA-21en
dc.type.driverconferenceObjecten
dc.type.statusPeer-revieweden
dc.type.versionpublishedVersionen
sync.item.dbidVAV-161434en
sync.item.dbtypeVAVen
sync.item.insts2025.02.03 15:49:55en
sync.item.modts2025.01.17 18:49:36en
thesis.grantorVysoké učení technické v Brně. Středoevropský technologický institut VUT. Chytré nanonástrojecs
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