Doubling Throughput of a Real-Time PCR

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Ahrberg, Christian D.
Neužil, Pavel

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Mark

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Macmillan Publishers

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Abstract

We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel
We added an intercallating dye to probe-based real-time PCR. One set of oligonucleotides had FAM probe while the second one was without. We were able to extract two DNA amplification curves using a single fluorescence channel

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Scientific Reports. 2015, vol. 15, issue 10, p. 1-10.
http://www.nature.com/articles/srep12595

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Peer-reviewed

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en

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Except where otherwised noted, this item's license is described as Creative Commons Attribution 4.0 International
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