Degradation of biogenic amines and in vitro evaluation of ruminal parameters of the ruminal fluid of Charolais sheep
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Date
2020-07-15
Authors
Koláčková, Ivana
Skládanka, Jiří
Skaličková, Sylvie
Horký, Pavel
Cernei, Natalia Vladimirovna
Lacková, Zuzana
Třináctý, Jiří
Adam, Vojtěch
0000-0002-8259-1566Advisor
Referee
Mark
Journal Title
Journal ISSN
Volume Title
Publisher
Sociedade Brasileira de Zootecnia
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Abstract
In this study, we reported the evaluation of ruminal parameters and the rate and degree of degradation of biogenic amines (BA) in vitro, using ruminal fluid from sheep fed two different diets (meadow hay and silage of Medicago sativa L.). Herein, the Charolais sheep breed with a ruminal cannula was used to gather samples. Multiple rumen parameters were evaluated after 10 days of the feeding period. Samples were processed using fermenters inoculated by BA (histamine, tyramine, putrescine, cadaverine, spermine, and spermidine) during 8-h fermentation. Levels of BA were determined using ion-exchange chromatography, and volatile fatty acids (VFA) were determined using gas chromatography. More rapid BA degradation was noticed in hay than in haylage. Overall, VFA levels increased with duration of the fermentation, while pH lowered. The rapid increase was observed after 4 h of fermentation. Ammonia concentration had a decreasing trend in silage and increasing in the hay. Hypotheses on BA degradation were accepted. Important knowledge of the development of ruminal parameters in time was obtained, and based on gathered information, it is possible to say that other parameters and their impacts on BA in rumen need to be examined further.
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Citation
Revista Brasileira de Zootecnia. 2020, vol. 49, issue 1, p. 1-9.
https://www.rbz.org.br/article/degradation-of-biogenic-amines-and-in-vitro-evaluation-of-ruminal-parameters-of-the-ruminal-fluid-of-charolais-sheep/
https://www.rbz.org.br/article/degradation-of-biogenic-amines-and-in-vitro-evaluation-of-ruminal-parameters-of-the-ruminal-fluid-of-charolais-sheep/
Document type
Peer-reviewed
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en