My_Umi_Tool: Processing Of Unique Molecular Identifiers Without Mapping To A Reference Genome

Abstract

The presented paper describes a novel algorithm for processing unique molecular identifiers (UMIs) without mapping to a reference genome. For the past few years, there has been a rapid rise in the use of sequencing technologies and progress in bioinformatics tools. However, available tools for processing UMIs are usually relative computationally demanding and time-consuming which led us to design and develop a new algorithm as well as to implement some available software to provide an effective estimation of the total number of unique molecules. My_UMI_tool performs four major steps: preprocessing of an input file, clustering by UMIs, clustering by sequence similarity, and marking duplicates to generate final file.