MARŠÍKOVÁ, B. Trojrozměrné zobrazování v holografickém mikroskopu pomocí koherenční brány [online]. Brno: Vysoké učení technické v Brně. Fakulta strojního inženýrství. 2018.

Posudek vedoucího

Chmelík, Radim

Cíle práce v oblasti trojrozměrného zobrazování holografickou mikroskopií s koherenční branou byly adekvátním postupem zcela splněny. Studentka si osvojila teoretický základ práce a dosáhla samostatně původních významných výsledků zejména v konstrukční a experimentální oblasti. Mimo jiné se významně podílela na provedení elektromagnetických simulací, návrhu konstrukčních úprav a výrobě modelového vzorku, samostatně experimentálně ověřila možnost zvýšení numerické apertury osvětlení a odpovídající zlepšení příčné i podélné rozlišovací schopnosti mikroskopu. Tyto výsledky budou základem pro další výzkum. Práce je graficky výborně zpracována, určitý prostor pro zlepšení vidím ve formulační oblasti a v uspořádání práce.

Posudek oponenta

Heintzmann, Rainer

The goals of the thesis were: 1. Determination of sample-details, axial localization dependence on the numerical aperture of condenser lenses by numerical simulation Barbora Maršíková presented very convincing high quality numerical simulations. She did get some help from a colleague with this, according to her report. 2. Design and realization of the microscope adaptation for the use of condenser optics with higher numerical aperture and lower optical aberrations. The thesis discusses multiple steps of improving and adapting the system to these requirements. Based on these experiments, a final configuration was chosen. 3. Experimental verification of simulation results This most difficult step of the thesis was also performed well. However, it did not end with a quantitative comparison but rather a qualitative confirmation of the results. Overall the requirements and objectives were fulfilled very well. 2. Procedure and extent of the solution, adequacy of the methods used Her thesis progresses in a very logical way and the methods used were adequate. In addition adequate suggestions for future improvements (different material, new condenser design) were given. The only slight critique is a missing quantification of the obtained results (curves and fits to obtain FWHM or alike). 3. Scholarly contribution and originality The topic is quite a difficult one from the theoretical as well as the experimental side. The presented master thesis shows a good degree of originality in the task as well as the solutions and suggestions for the future, in combination with a thorough understanding of the topic. 4. Ability to interpret the results achieved and draw conclusions The interpretations are all accurate in my understanding and the correct conclusions are drawn from them. 5. Applicability of results in practice or theory The obtained results are applicable for future systems, further system development and research. Also the summary of theory and the numerical simulations will be useful for future research. She also showed a good application of her knowledge and understanding of theory to achieve these simulations as well as a good experience in optics to be able to align such a complex experimental setup. 6. Logical arrangement of the thesis and its formal layout The logical arrangement of the thesis is well thought through. After an introduction to the system and the necessary theoretical, she first concentrates on the description of the production of the reference sample, followed by the improvement of the condenser design to end with the final measurements, comparison, interpretation and conclusion. 7. Graphic layout, style used and language level The graphic layout and style is very good. There are only very few mistakes in equations. The language level, considering that it was written in English, is generally very good, but some necessary articles (“a”, “the”) are missing. It should be noted that for official publications and the PhD thesis the copying of figures from other publications, as seen here in the introduction, is not acceptable for copyright reasons. 8. Work with literature including citations All the related work is adequately and in my view correctly cited. This thesis is a very good example of scientific working and drawing conclusions. Even if the results were not ideal, it is seen that the candidate understood what she was doing very well and progressed by steadily improving the setup and simulations. As a comment: the term “axial localization” which was already used in the tasks given to the student, was not a great choice as this is easily (wrongly) interpreted to mean “axial localization precision”. However it seems to refer to the extend over which a refractive index distortion influences the image. “Axial extend of the coherent amplitude PSF” or alike, seem to be a better fitting term.

eVSKP id 109699